TEDA Institute of Biological Sciences & Biotechnology, Nankai University, TEDA, Tianjin 300457, PR China.
The Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Tianjin 300071, PR China.
Future Microbiol. 2018 Jun 1;13:757-769. doi: 10.2217/fmb-2017-0256. Epub 2018 May 31.
Determination of the effects of BirA on transcription and virulence in enterohemorrhagic Escherichia coli (EHEC) O157:H7.
MATERIALS & METHODS: The effect of BirA on EHEC O157:H7 gene expression and phenotypes was assessed by RNA-seq combined with adherence, quantitative biofilm and survival assays.
Many genes associated with virulence, amino acid synthesis and transport, and zinc transport were upregulated, whereas genes encoding stress proteins were downregulated in ΔbirA::km+Ac_birA. Accordingly, ΔbirA::km+Ac_birA adhesion to Caco-2 cells, biofilm formation and survival during oxidative stress were higher, whereas its survival during heat shock was lower than that of the wild-type.
This study demonstrates the wide-ranging regulatory functions of BirA, especially its role in controlling virulence and stress responses in EHEC O157:H7. [Formula: see text].
确定 BirA 对肠出血性大肠杆菌（EHEC）O157：H7 转录和毒力的影响。
通过 RNA-seq 结合黏附、定量生物膜和存活测定来评估 BirA 对 EHEC O157：H7 基因表达和表型的影响。
许多与毒力、氨基酸合成和转运以及锌转运相关的基因上调，而编码应激蛋白的基因下调在 ΔbirA::km+Ac_birA 中。相应地，ΔbirA::km+Ac_birA 对 Caco-2 细胞的黏附、生物膜形成和氧化应激存活能力增强，而其在热激下的存活能力低于野生型。
本研究表明 BirA 具有广泛的调节功能，特别是在控制 EHEC O157：H7 毒力和应激反应方面的作用。[公式：见文本]。
