Department of General Surgery, Chang Gung Memorial Hospital at Linkou, Chang Gung University College of Medicine, Taoyuan, Taiwan.
Biomed Res Int. 2018 Apr 23;2018:6197152. doi: 10.1155/2018/6197152. eCollection 2018.
Clinically severe infection-related inflammation after major liver resection may cause hyperbilirubinemia. This study aims to clarify the impact of bacterial infection and endotoxins on the hepatobiliary transporter system and to explore possible mechanisms of endotoxin-related postoperative hyperbilirubinemia.
Mice that underwent major hepatectomy with removal of at least 70% of liver volume were exposed to lipopolysaccharide (LPS) at different dosages. Subsequently, hepatobiliary transporter compounds related to bile salt excretion were further investigated.
The expression of genes related to hepatobiliary transporter compounds was not significantly different in the liver tissue of mice after major hepatectomy and LPS exposure. However, bile salt export pump (BSEP) protein expression within the liver tissue of mice treated with LPS after major hepatectomy was relatively weaker and was even further reduced in the high-dose LPS group. The formation of antibodies against the BSEP in response to endotoxin exposure was also detected.
This study illustrates a possible mechanism whereby the dysfunction of hepatobiliary transporter systems caused by endotoxin-induced autoantibodies may be involved in the development of postoperative jaundice associated with bacterial infection after major hepatectomy.
肝叶切除术后严重的感染相关炎症可能导致高胆红素血症。本研究旨在阐明细菌感染和内毒素对肝胆转运系统的影响，并探讨内毒素相关术后高胆红素血症的可能机制。
将至少 70%肝体积切除的肝叶切除术的小鼠暴露于不同剂量的脂多糖（LPS）。随后，进一步研究了与胆汁盐排泄相关的肝胆转运化合物。
肝叶切除和 LPS 暴露后，小鼠肝组织中与肝胆转运化合物相关的基因表达无明显差异。然而，肝组织中 LPS 处理后的 BSEP 蛋白表达相对较弱，高剂量 LPS 组的表达进一步降低。还检测到针对内毒素暴露形成的 BSEP 抗体。
本研究阐明了一种可能的机制，即内毒素诱导的自身抗体引起的肝胆转运系统功能障碍可能参与了肝叶切除术后细菌感染相关的术后黄疸的发生。
