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FBXO5 两个转录本促进人牙周膜间充质干细胞的迁移和成骨分化。

Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells.

机构信息

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu, China.

Department of Dental Implantology, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, Jiangsu, China.

出版信息

Biomed Res Int. 2018 Apr 19;2018:7849294. doi: 10.1155/2018/7849294. eCollection 2018.

DOI:10.1155/2018/7849294
PMID:29850565
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5933072/
Abstract

OBJECTIVES

Enhanced migration and osteogenic differentiation of mesenchymal stem cells (MSCs) are beneficial for MSC-mediated periodontal tissue regeneration, a promising method for periodontitis treatment. FBXO5, a member of the F-box protein family, is involved in the osteogenic differentiation of MSCs. Here, we investigated the effect of FBXO5 on human periodontal ligament stem cells (hPDLSCs).

MATERIALS AND METHODS

hPDLSCs were isolated from periodontal ligament tissue. Lentivirus FBXO5 shRNA was used to silence FBXO5 expression. Two transcripts of FBXO5 were overexpressed and transduced into hPDLSCs via retroviral infection. Migration and osteogenic differentiation of hPDLSCs were evaluated using the scratch migration assay, alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, western blotting, and real-time polymerase chain reaction.

RESULTS

The expression of FBXO5 was upregulated after osteogenic induction in hPDLSCs. FBXO5 knockdown attenuated migration, inhibited ALP activity and mineralization, and decreased RUNX2, OSX, and OCN expression, while the overexpression of two transcript isoforms significantly accelerated migration, enhanced ALP activity and mineralization, and increased RUNX2, OSX, and OCN expression in hPDLSCs.

CONCLUSIONS

Both isoforms of FBXO5 promoted the migration and osteogenic differentiation potential of hPDLSCs, which identified a potential target for improving periodontal tissue regeneration.

摘要

目的

增强间充质干细胞（MSCs）的迁移和成骨分化有利于 MSC 介导的牙周组织再生，这是一种有前途的治疗牙周炎的方法。FBXO5 是 F-box 蛋白家族的一员，参与 MSC 的成骨分化。在这里，我们研究了 FBXO5 对人牙周膜干细胞（hPDLSCs）的影响。

材料和方法

从牙周膜组织中分离 hPDLSCs。使用慢病毒 FBXO5 shRNA 沉默 FBXO5 表达。通过逆转录病毒感染过表达和转导 FBXO5 的两种转录本。通过划痕迁移实验、碱性磷酸酶（ALP）活性、ALP 染色、茜素红染色、western blot 和实时聚合酶链反应评估 hPDLSCs 的迁移和成骨分化。

结果

hPDLSCs 成骨诱导后 FBXO5 的表达上调。FBXO5 敲低减弱了迁移，抑制了 ALP 活性和矿化，并降低了 RUNX2、OSX 和 OCN 的表达，而两种转录本异构体的过表达显著加速了 hPDLSCs 的迁移，增强了 ALP 活性和矿化，并增加了 RUNX2、OSX 和 OCN 的表达。

结论

FBXO5 的两种异构体都促进了 hPDLSCs 的迁移和成骨分化潜能，这为改善牙周组织再生确定了一个潜在的靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/40f56cf4101a/BMRI2018-7849294.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/5a689d812847/BMRI2018-7849294.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/5d1bd9fc16ee/BMRI2018-7849294.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/bda4ed61189a/BMRI2018-7849294.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/40a532fbae2c/BMRI2018-7849294.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/40f56cf4101a/BMRI2018-7849294.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/5a689d812847/BMRI2018-7849294.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/5d1bd9fc16ee/BMRI2018-7849294.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/bda4ed61189a/BMRI2018-7849294.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/40a532fbae2c/BMRI2018-7849294.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31f9/5933072/40f56cf4101a/BMRI2018-7849294.005.jpg

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2
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Stem Cells Int. 2017;2017:3028647. doi: 10.1155/2017/3028647. Epub 2017 Oct 29.
3
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4
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5
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6
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7
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