Mezey E, Kiss J Z, Mueller G P, Eskay R, O'Donohue T L, Palkovits M
Brain Res. 1985 Mar 4;328(2):341-7. doi: 10.1016/0006-8993(85)91046-7.
Rat hypothalamic nuclei were removed and assayed for adrenocorticotropic hormone (ACTH), beta-endorphin (beta-END) and alpha-melanocyte-stimulating hormone (alpha-MSH) content by radioimmunoassay, from the same samples. We also performed immunostaining for these 3 pro-opiomelanocortin (POMC) derived peptides on paraffin embedded serial sections of the hypothalamus. Areas known to project to the external zone of the median eminence receive a dense POMC innervation while those projecting to the posterior pituitary are not innervated. In addition, hypothalamic areas previously suggested to project to medullary autonomic centers are densely innervated. This innervation pattern may provide the morphological basis for the involvement of POMC peptides in neuroendocrine and autonomic functions. The biochemical data raise the possibility that the POMC precursor is processed differently in various brain regions.
切除大鼠下丘脑核，并通过放射免疫分析法对相同样本检测促肾上腺皮质激素（ACTH）、β-内啡肽（β-END）和α-黑素细胞刺激素（α-MSH）的含量。我们还对下丘脑石蜡包埋连续切片上的这三种源自阿片-促黑素细胞皮质素原（POMC）的肽进行了免疫染色。已知投射到正中隆起外侧区的区域接受密集的POMC神经支配，而投射到垂体后叶的区域则没有神经支配。此外，先前提示投射到髓质自主神经中枢的下丘脑区域有密集的神经支配。这种神经支配模式可能为POMC肽参与神经内分泌和自主神经功能提供形态学基础。生化数据增加了POMC前体在不同脑区加工方式不同的可能性。
