National Institute of Plant Genome Research, Jawaharlal Nehru University, Aruna Asaf Ali Marg, New Delhi 110067, India.
National Institute of Plant Genome Research, Jawaharlal Nehru University, Aruna Asaf Ali Marg, New Delhi 110067, India.
J Proteomics. 2018 Jul 15;183:45-57. doi: 10.1016/j.jprot.2018.05.013. Epub 2018 May 29.
Grasspea, a stress-resilient pulse crop, has largely remained outside the realm of phytochemical and functional genomics analyses despite its high nutritional significance. To unravel the intervarietal variability in nutrient acquisition of grasspea, we conducted a series of physicochemical experiments using two cultivated varieties, LP-24 and Prateek. The analyses revealed high percentage of starch, cellulose, peroxides, carotenoids, phytic acid and minerals in cv. LP-24, whereas large amounts of protein, soluble carbohydrates and antioxidants in Prateek. To dissect the mechanism of stress tolerance, 3-week-old seedlings of cv. LP-24 and Prateek were afflicted with dehydration for a period of 144 h. The physicochemical indices indicated better adaptation in cv. LP-24, with high abundance of proline, phenolics and flavonoids. Dehydration-responsive proteome landscape of cv. LP-24 revealed 152 proteins with variance at a statistically 94% significance level. The comparative proteomics analysis led to the identification of 120 dehydration-responsive proteins (DRPs), most of which were associated with carbohydrate metabolism, amino acid synthesis, antioxidant reactions and cell defense. We report, for the first time, the dehydration-induced proteome landscape of grasspea, whose genome is yet to be sequenced. The results provide unique insights into variety-specific nutrient acquisition attributes and dehydration-tolerance of grasspea.
Grasspea is a great source of protein and antioxidants with nitrogen fixing ability, besides its tolerance to multivariate environmental stress as compared to major legume species. This represents the first report on nutrient profile and health-promoting attributes of grasspea. The cultivars under study are nutritionally enriched that possess high protein, amino acids and health-promoting factors and may therefore be projected as a vital part of a healthy diet. Grasspea is known for its hardy nature, water-use efficiency and efficacy as a stress-tolerant pulse. Further, this study portrays the dehydration-responsive proteomic landscape of grasspea. The proteomics analyses provide crucial insights into the dehydration response, presumably orchestrated by proteins belonging to an array of functional classes including photosynthesis, protein and RNA metabolism, protein folding, antioxidant enzymes and defense. The interplay of the differentially regulated proteins might aid in reinforcing the mechanisms of dehydration avoidance and/or tolerance.
尽管草豌豆具有很高的营养价值，但它作为一种抗逆性很强的豆类作物，在植物化学和功能基因组学分析方面基本上仍未得到广泛研究。为了揭示草豌豆品种间养分获取的变异性，我们使用两个栽培品种 LP-24 和 Prateek 进行了一系列物理化学实验。分析表明，LP-24 品种的淀粉、纤维素、过氧化物、类胡萝卜素、植酸和矿物质含量百分比高，而 Prateek 品种的蛋白质、可溶性碳水化合物和抗氧化剂含量大。为了剖析其耐受胁迫的机制，我们对 LP-24 和 Prateek 品种的 3 周龄幼苗进行了为期 144 小时的脱水处理。物理化学指标表明，LP-24 品种适应性更好，脯氨酸、酚类和类黄酮含量丰富。LP-24 品种的脱水响应蛋白质组景观揭示了 152 种在统计学上具有 94%显著性水平差异的蛋白质。比较蛋白质组学分析导致鉴定出 120 种脱水响应蛋白（DRP），其中大多数与碳水化合物代谢、氨基酸合成、抗氧化反应和细胞防御有关。我们首次报道了草豌豆的脱水诱导蛋白质组景观，而其基因组尚未测序。研究结果为草豌豆品种特异性养分获取特性和耐旱性提供了独特的见解。
