National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi, 110067, India.
National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi, 110067, India.
Phytochemistry. 2022 Oct;202:113296. doi: 10.1016/j.phytochem.2022.113296. Epub 2022 Jul 20.
Plant secretomics has been especially important in understanding the molecular basis of plant development, stress resistance and biomarker discovery. In addition to sharing a similar role in maintaining cell metabolism and biogenesis with the animal secretome, plant-secreted proteins actively participate in signaling events crucial for cellular homeostasis during stress adaptation. However, investigation of the plant secretome remains largely overlooked, particularly in pulse crops, demanding urgent attention. To better understand the complexity of the secretome, we developed a reference map of a stress-resilient orphan legume, Lathyrus sativus (grasspea), which can be utilized as a potential proteomic resource. Secretome analysis of L. sativus led to the identification of 741 nonredundant proteins belonging to a myriad of functional classes, including antimicrobial, antioxidative and redox potential. Computational prediction of the secretome revealed that ∼29% of constituents are predicted to follow unconventional protein secretion (UPS) routes. We conducted additional in planta analysis to determine the localization of two secreted proteins, recognized as cell surface residents. Sequence-based homology comparison revealed that L. sativus shares ∼40% of the constituents reported thus far from in vitro and in planta secretome analysis in model and crop species. Significantly, we identified 571 unique proteins secreted from L. sativus involved in cell-to-cell communication, organ development, kinase-mediated signaling, and stress perception, among other critical roles. Conclusively, the grasspea secretome participates in putative crosstalk between genetic circuits that regulate developmental processes and stress resilience.
植物分泌组学在理解植物发育、抗逆性和生物标志物发现的分子基础方面尤为重要。除了与动物分泌组在维持细胞代谢和生物发生方面发挥相似作用外，植物分泌蛋白还积极参与应激适应过程中细胞内稳态的关键信号事件。然而，植物分泌组的研究在很大程度上仍被忽视，特别是在豆类作物中，这需要引起紧急关注。为了更好地理解分泌组的复杂性，我们开发了一个抗逆性孤雌豆科植物——豌豆（Lathyrus sativus）的参考图谱，它可以作为潜在的蛋白质组资源加以利用。对 L. sativus 的分泌组分析导致鉴定出 741 种非冗余蛋白，这些蛋白属于多种功能类别，包括抗菌、抗氧化和氧化还原潜力。分泌组的计算预测表明，约 29%的组成部分被预测遵循非常规蛋白分泌（UPS）途径。我们进行了额外的体内分析，以确定两种分泌蛋白的定位，这两种蛋白被认为是细胞表面居民。基于序列的同源性比较表明，豌豆与目前从模型和作物物种的体外和体内分泌组分析中报告的约 40%的组成部分具有相似性。值得注意的是，我们鉴定出 571 种从 L. sativus 分泌的独特蛋白，这些蛋白参与细胞间通讯、器官发育、激酶介导的信号转导以及应激感知等关键作用。总之，豌豆的分泌组参与了调节发育过程和应激弹性的遗传回路之间的潜在串扰。
