Department of Dental Materials and Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of Korea.
Department of Dental Materials and Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of Korea.
Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:113-118. doi: 10.1016/j.msec.2018.04.060. Epub 2018 Apr 21.
In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF). The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF showed a homogeneous distribution of spherical nano-clusters. The hydrophilicity of the coatings was increased by performing NaOH post-treatment of CaP/CS composites, which also affected apatite formation. Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell adhesion and osteogenic properties of the mesoporous CaP/CS composite were evaluated by SEM and ALPase assay, respectively. This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites. These results indicate that this CaP/CS composite could be a promising candidate for bone tissue engineering.
为了提高壳聚糖的生物相容性和生物活性，通过在改良的模拟体液（m-SBF）中孵育，将羟基磷灰石原位结合到钛基体上的旋涂壳聚糖层中。在 m-SBF 中制备的磷酸钙/壳聚糖（CaP/CS）复合材料表现出球形纳米簇的均匀分布。通过对 CaP/CS 复合材料进行 NaOH 后处理，提高了涂层的亲水性，这也影响了磷灰石的形成。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐测定法研究人成骨样 MG-63 细胞的细胞反应来评估涂层的生物相容性。通过 SEM 和 ALPase 测定分别评估了介孔 CaP/CS 复合材料的细胞黏附和成骨性能。这项体外研究表明，在纳米结构 CaP/CS 复合材料上可以改善细胞黏附和分化。这些结果表明，这种 CaP/CS 复合材料可能是骨组织工程的有前途的候选材料。
