Jaramillo-Martínez S, Vargas-Requena C, Rodríguez-Gónzalez C, Hernández-Santoyo A, Olivas-Armendáriz I
Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente del PRONAF y Estocolmo, C.P.32320, Cd. Juárez, Chihuahua, Mexico.
Instituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez, Ave. Del Charro #610 norte, Col. Partido Romero, C.P.32320, Cd. Juárez, Chihuahua, Mexico.
Heliyon. 2019 Aug 27;5(8):e02252. doi: 10.1016/j.heliyon.2019.e02252. eCollection 2019 Aug.
Biomineralization is the process by which diverse organisms have the capacity to create heterogeneous accumulations, derived from organic and inorganic compounds that induce the process of mineral formation. An example of this can be seen an extrapallial protein (EP) of , which is responsible for carrying out the biomineralization process. In order to determine their ability to perform the biomineralization process, EP protein was absorbed and mixed in chitosan scaffolds which were tested in simulated physiological fluid. The materials were analyzed by FTIR spectroscopy, field emission scanning electron microscopy-energy-dispersive electron X-ray spectroscopy andX-ray diffraction. Results confirmed that the EP protein stimulates the rapid growth of biological apatite on the chitosan scaffolds. The mixing method favored more the apatite growth as well as the formation of second nucleation sites than the immersion method.
生物矿化是一个过程，通过这个过程，不同的生物体有能力产生由有机和无机化合物衍生而来的异质聚积物，这些化合物会引发矿物形成过程。这方面的一个例子可见于 的一种外套膜外蛋白质（EP），它负责执行生物矿化过程。为了确定它们执行生物矿化过程的能力，将EP蛋白吸附并混入壳聚糖支架中，然后在模拟生理流体中进行测试。通过傅里叶变换红外光谱、场发射扫描电子显微镜-能量色散电子X射线光谱和X射线衍射对材料进行分析。结果证实，EP蛋白刺激壳聚糖支架上生物磷灰石的快速生长。与浸泡法相比，混合法更有利于磷灰石的生长以及二次成核位点的形成。
