Polymer Materials Research Department, Advanced Technologies and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box: 21934, Alexandria, Egypt; Laboratory of Bioorganic Chemistry of Drugs, Centre of Experimental Medicine, Institute of Experimental Pharmacology and Toxicology, 84104 Bratislava, Slovakia.
Laboratory of Bioorganic Chemistry of Drugs, Centre of Experimental Medicine, Institute of Experimental Pharmacology and Toxicology, 84104 Bratislava, Slovakia.
Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:227-235. doi: 10.1016/j.msec.2018.04.053. Epub 2018 Apr 19.
A novel wound healing material composed of chitosan (Ch) and hyaluronan (HA) boosted with edaravone (Ed) as an anti-inflammatory drug was developed. The fabricated membranes were verified using FT-IR, and the thermal properties were estimated employing TGA instrument. Moreover, Physical characterizations of the prepared membranes demonstrated a decrease in the membrane wettability, whereas an increase in membrane roughness was monitored due to the effect of edaravone supplementation. A comparative study of free-radical scavenging activity of edaravone itself was carried out by two in vitro approaches: uninhibited/inhibited hyaluronan degradation and decolorization of ABTS methods in normal and simulated inflammation condition (acidic condition). Accordingly, the scavenging activity of edaravone was significantly diminished to OH and peroxy-/alkoxy-type radicals in acidic conditions in compared to the neutral reactions. The biochemical studies evidenced the haemocompatibility of the examined membranes. The consequence of membranes composed of Ch/HA/Ed on the wound healing of the rat's skin was studied, and the macroscopic and microscopic investigations revealed remarkable healing at 21 day post-surgery compared with injuries treated with cotton gauze as a negative control in addition to Ch/HA membrane without edaravone. For these reasons, the Ch/HA/Ed membrane could be implemented as wound dressing material.
一种新型的创伤愈合材料，由壳聚糖（Ch）和透明质酸（HA）组成，并添加了作为抗炎药物的依达拉奉（Ed）。使用傅里叶变换红外光谱（FT-IR）对所制备的膜进行了验证，并使用热重分析仪（TGA）评估了其热性能。此外，对所制备的膜进行了物理特性分析，结果表明由于依达拉奉的添加，膜的润湿性降低，而膜粗糙度增加。通过两种体外方法：未抑制/抑制透明质酸降解和 ABTS 法在正常和模拟炎症条件（酸性条件）下的褪色，对依达拉奉本身的自由基清除活性进行了比较研究。因此，与中性反应相比，依达拉奉在酸性条件下对 OH 和过氧/烷氧基型自由基的清除活性显著降低。生化研究证明了所研究的膜具有良好的血液相容性。研究了由 Ch/HA/Ed 组成的膜对大鼠皮肤创伤愈合的影响，宏观和微观研究结果表明，与作为阴性对照的棉纱布相比，在术后 21 天，用 Ch/HA 膜（不含依达拉奉）治疗的伤口愈合有显著改善。基于这些原因，Ch/HA/Ed 膜可以用作伤口敷料材料。
