Polymer Materials Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box, 21934 Alexandria, Egypt.
Chemistry Department, Polymer Research Group, Faculty of Science, University of Tanta, Tanta 31527, Egypt.
Int J Biol Macromol. 2022 Jun 30;211:94-106. doi: 10.1016/j.ijbiomac.2022.05.061. Epub 2022 May 11.
Herein, wound dressing membranes based on covalently linked Chitosan (Ch) to Gelatin (GE) via Glutaraldehyde (GA) to have (Ch-GA-GE) copolymer have been developed. In addition, Anisaldehyde (An) was immobilized onto Ch-GA-GE to has An-(Ch-GA-GE) membrane. The changes of the Ch-GA-GE membranes wettability, from 26 ± 1.3° to 45.3 ± 2.27° of the An-(Ch-GA-GE) copolymer membrane, indicating the reduction of copolymers hydrophilicity. The thermal characterization was done using TGA and DSC, while the morphological analysis was done using SEM. The antibacterial properties were assessed against four bacterial strains (P. aeruginosa, S. aureus, Streptococcus, and E. coli). In-vitro evaluation of the fabricated membranes to be used as wound dressings was investigated by measuring their hemocompatibility, cytotoxicity, and biodegradability. Finally, the in-vivo assessment of the developed membranes to encourage skin regeneration was assessed utilizing adult Wistar albino rats. The results illustrated that the An-(Ch-GA-GE) copolymer membranes significantly enhanced the rat's full-thickness injuries, as monitored by reducing the wound region. Furthermore, histological analyses of the injuries covered with An-(Ch-GA-GE) membranes demonstrated a notable re-epithelialisation contrasted with wounds treated with the cotton gauze Ch-GA-GE membranes dressings proving the efficiency of Anisaldehyde. Those findings indicate that the An-(Ch-GA-GE) membrane has considerable potential for wound healing and skin regeneration.
在此，通过戊二醛（GA）将壳聚糖（Ch）共价连接到明胶（GE）上，开发了基于壳聚糖（Ch）-GA-明胶（GE）的（Ch-GA-GE）共聚物的伤口敷料膜。此外，将茴香醛（An）固定在 Ch-GA-GE 上，得到 An-（Ch-GA-GE）膜。Ch-GA-GE 膜的润湿性发生变化，从 26±1.3°变为 45.3±2.27°的 An-（Ch-GA-GE）共聚物膜，表明共聚物的亲水性降低。使用 TGA 和 DSC 进行热特性分析，使用 SEM 进行形态分析。采用四种细菌菌株（铜绿假单胞菌、金黄色葡萄球菌、链球菌和大肠杆菌）评估抗菌性能。通过测量其血液相容性、细胞毒性和生物降解性，研究了所制备的用作伤口敷料的膜的体外评估。最后，利用成年 Wistar 白化大鼠评估了开发的促进皮肤再生的膜的体内评估。结果表明，An-（Ch-GA-GE）共聚物膜通过减少伤口区域，显著促进了大鼠的全层损伤。此外，用 An-（Ch-GA-GE）膜覆盖的伤口的组织学分析表明，与用 Ch-GA-GE 棉纱布敷料治疗的伤口相比，明显的再上皮化，证明了茴香醛的效率。这些发现表明，An-（Ch-GA-GE）膜在伤口愈合和皮肤再生方面具有很大的潜力。
