Polymer Materials Research Department, Advanced Technology and New Material Research Institute (ATNMRI), Scientific Research and Technological Applications City (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt.
Medical Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Scientific Research and Technological Applications City (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt.
Int J Biol Macromol. 2018 May;111:649-659. doi: 10.1016/j.ijbiomac.2018.01.040. Epub 2018 Jan 12.
In this study, new hydrogel membranes were developed based on hydroxyethyl cellulose (HEC) supplemented with tungsten oxide for further implementing in wound treatment. HEC hydrogel membranes were fabricated and crosslinked using citric acid (CA). Various tests were carried out including FTIR, XRD, porosity measurements, swelling, mechanical properties, gel fraction, and thermal gravimetric analysis to evaluate the efficiency of the prepared membranes as wound dressing material. In addition, wound healing activity of the examined membranes for human dermal fibroblast cell line was investigated employing in vitro scratching model. Furthermore, the potency of the prepared membranes to suppress wound complications was studied via determination of their anti-inflammatory and antibacterial activities exploiting MTT, ELISA, and disk agar diffusion methods. The results demonstrated that the HEC hydrogel membranes revealed an anti-inflammatory and antibacterial efficacy. Moreover, HEC improved the safety of tungsten oxide toward normal human cells (white blood cells and dermal fibroblast). Furthermore, HEC membranes loaded with WO revealed the highest activities against Salmonella sp. pursued by P. aeruginosa in compared with the negative HEC hydrogel membrane. The current approach corroborated that HEC amended by tungsten oxide could be applied as a promising safe candidate for wound dressing material.
在这项研究中，开发了基于羟乙基纤维素（HEC）的新型水凝胶膜，并添加了氧化钨，以进一步应用于伤口治疗。使用柠檬酸（CA）制备和交联 HEC 水凝胶膜。进行了各种测试，包括 FTIR、XRD、孔隙率测量、溶胀、机械性能、凝胶分数和热重分析，以评估所制备的膜作为伤口敷料材料的效率。此外，通过体外划痕模型研究了所检查的膜对人皮肤成纤维细胞系的伤口愈合活性。此外，通过 MTT、ELISA 和圆盘琼脂扩散方法测定其抗炎和抗菌活性，研究了制备的膜抑制伤口并发症的能力。结果表明，HEC 水凝胶膜具有抗炎和抗菌功效。此外，HEC 提高了氧化钨对正常人体细胞（白细胞和皮肤成纤维细胞）的安全性。此外，载有 WO 的 HEC 膜对沙门氏菌的活性最高，其次是铜绿假单胞菌，而阴性 HEC 水凝胶膜则较低。目前的方法证实，用氧化钨修饰的 HEC 可用作有前途的伤口敷料材料的安全候选物。
