Dea S, Begin M E, Archambault D, Elazhary M A, Roy R S
Cornell Vet. 1985 Apr;75(2):307-18.
Rotaviruses were isolated following cell culture of the intestinal contents of four non-diarrheic calves. The four isolates were serially propagated in MDBK and BSC-1 cells in the presence of trypsin and produced rotavirus particles morphologically similar to those found associated with diarrhea. They were antigenically related to the Nebraska calf rotavirus (Norden strain) as investigated by immunofluorescence. Three isolates could be distinguished from the reference Nebraska rotavirus by their thermal stability and/or their differential responses to intestinal neutralizing antibodies. Two isolates produced on BSC-1 cells plaques significantly different in size from the reference strain, No significant genomic variations were detected among the isolates.
从四头无腹泻小牛的肠道内容物进行细胞培养后分离出轮状病毒。这四株分离株在胰蛋白酶存在的情况下于MDBK和BSC - 1细胞中连续传代培养，产生了形态上与腹泻相关的轮状病毒颗粒相似的病毒颗粒。通过免疫荧光研究发现，它们与内布拉斯加小牛轮状病毒（诺登株）存在抗原相关性。通过热稳定性和/或对肠道中和抗体的不同反应，三株分离株可与参考内布拉斯加轮状病毒区分开来。在BSC - 1细胞上产生的两株分离株形成的噬菌斑大小与参考菌株有显著差异，在分离株中未检测到明显的基因组变异。
