通过蚀斑试验对牛轮状病毒分离株进行比较研究。
Comparative study of bovine rotavirus isolates by plaque assay.
作者信息
Archambault D, Roy R S, Dea S, Elazhary M A
出版信息
Can J Comp Med. 1984 Jul;48(3):286-9.
Rotaviruses were isolated on BSC-1 cells from counterimmunoelectrophoresis and/or electron microscopy positive intestinal contents from two asymptomatic and six diarrheic calves from Quebec. The plaque assay was performed using these lines and agar overlay medium containing trypsin and DEAE-dextran. This assay was used to compare the Quebec isolates to an attenuated American strain (NCDV) and another strain (TH) obtained from France. The NCDV strain produced plaques that were significantly larger than those produced by the TH strain. Three Quebec isolates produced plaques similar in size to TH strain, one isolate was similar to NCDV strain and another isolate produced larger plaques than those of both NCDV and TH strains. The other isolates induced the production of plaques that were not significantly different from those of NCDV or TH strains.
从魁北克的两头无症状和六头腹泻小牛的免疫对流电泳和/或电子显微镜检查呈阳性的肠道内容物中，在BSC - 1细胞上分离出轮状病毒。使用这些细胞系以及含有胰蛋白酶和二乙氨基乙基葡聚糖的琼脂覆盖培养基进行噬斑测定。该测定用于将魁北克分离株与减毒的美国毒株（NCDV）和从法国获得的另一毒株（TH）进行比较。NCDV毒株产生的噬斑明显大于TH毒株产生的噬斑。三个魁北克分离株产生的噬斑大小与TH毒株相似，一个分离株与NCDV毒株相似，另一个分离株产生的噬斑比NCDV和TH毒株的都大。其他分离株诱导产生的噬斑与NCDV或TH毒株的噬斑没有显著差异。
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