Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy.
Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy.
Food Chem. 2018 Oct 30;264:241-249. doi: 10.1016/j.foodchem.2018.04.111. Epub 2018 Apr 26.
This study was carried out to develop an UHPLC-MS/MS analytical procedure, to determinate all isomers and isoforms of crocins of 42 saffron samples, with different origin, age and dried using different process conditions. A preliminary experimental design was applied to optimize the extraction of crocins; UHPLC-MS/MS conditions were set to obtain the best analytical performances in terms of sensitivity and selectivity. The optimised conditions allowed to determine ten crocins; their amount in samples was significantly different and affected by process, age and origin. Drying conditions influenced the crocins pattern and this was particularly evidenced in the more recently produced samples, with a clear separation between mild and high thermally treated samples. Principal Component Analysis of all crocins data allowed to discriminate samples based on origin (Italy vs. other countries) and age. Results confirm the feasibility of the use of crocins pattern as marker of quality and traceability of saffron.
本研究旨在开发一种 UHPLC-MS/MS 分析方法，以测定来自不同产地、不同陈化年限且采用不同干燥工艺的 42 个藏红花样品中的所有西红花苷异构体和同工型。采用初步实验设计来优化西红花苷的提取；UHPLC-MS/MS 条件的设定旨在获得最佳的灵敏度和选择性分析性能。优化后的条件允许测定十种西红花苷；它们在样品中的含量存在显著差异，并受到工艺、陈化年限和产地的影响。干燥条件影响西红花苷的图谱，这在最近生产的样品中表现得尤为明显，其中温和处理和高温处理的样品之间有明显的分离。对所有西红花苷数据的主成分分析允许根据产地（意大利与其他国家）和陈化年限对样品进行区分。研究结果证实了西红花苷图谱作为藏红花质量和可追溯性标志物的可行性。
