Gaffer Ghada Gamal, Elgawish Rania Abdelrahman, Abdelrazek Heba M A, Ebaid Hala M, Tag Hend M
Department of Zoology, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Toxicol Rep. 2018 Feb 9;5:296-301. doi: 10.1016/j.toxrep.2018.02.002. eCollection 2018.
Phytoestrogens have an impact on both animals and humans due to use of legumes in animal diets as well as the increase of vegetarian diets in some human populations. Phytoestrogens thought to have varieties of adverse effects, among which immune system was involved. The present study aimed to investigate the effect of prenatal exposure to dietary soy isoflavones on some immunological parameters in male albino rat offspring. The pregnant rats were divided to three groups (12/group). Control group (free soy isoflavones), low soy isoflavones group (6.5%) and high soy isoflavones group (26%). The male offspring cell-mediated immune response was determined using phytohemagglutinin (PHA) injection and the intumesce index which was calculated on postnatal day 50 (PND 50). At PND 50, blood samples were collected for interleukin 12 (IL-12), interferon γ (IFN-γ) and tumor necrosis factor α (TNF-α) determination. Spleen, thymus, and PHA injected footpads were fixed for histopathology. Intumesce index, IL-12, IFN-γ, spleen and thymus relative weights were significantly ( 0.05) decreased in offspring born to dams fed low and high dietary soy isoflavones. In contrary, TNF-α was significantly ( 0.05) increased in offspring born to dams fed high dietary soy isoflavones. Spleen of rats born to dams fed high dose of dietary soy isoflavones showed coagulative necrosis in white pulp. In conclusion, male offspring born to dams fed different levels of soy isoflavones showed marked immunosuppression after PHA stimulation. This effect was mediated through the reduced IFN-γ that interacts with the IL-12 production pathway.
由于动物日粮中使用豆类以及一些人群中素食饮食的增加，植物雌激素对动物和人类都有影响。植物雌激素被认为具有多种不良影响，其中涉及免疫系统。本研究旨在调查产前暴露于膳食大豆异黄酮对雄性白化大鼠后代某些免疫参数的影响。将怀孕大鼠分为三组（每组12只）。对照组（无大豆异黄酮）、低大豆异黄酮组（6.5%）和高大豆异黄酮组（26%）。在出生后第50天（PND 50），通过注射植物血凝素（PHA）并计算肿大指数来测定雄性后代的细胞介导免疫反应。在PND 50时，采集血样测定白细胞介素12（IL-12）、干扰素γ（IFN-γ）和肿瘤坏死因子α（TNF-α）。将脾脏、胸腺和注射PHA的足垫固定用于组织病理学检查。在喂食低剂量和高剂量膳食大豆异黄酮的母鼠所生后代中，肿大指数、IL-12、IFN-γ、脾脏和胸腺相对重量显著（P<0.05）降低。相反，在喂食高剂量膳食大豆异黄酮的母鼠所生后代中，TNF-α显著（P<0.05）升高。喂食高剂量膳食大豆异黄酮的母鼠所生大鼠的脾脏白髓出现凝固性坏死。总之，喂食不同水平大豆异黄酮的母鼠所生雄性后代在PHA刺激后表现出明显的免疫抑制。这种作用是通过与IL-12产生途径相互作用的IFN-γ减少介导的。
