Influence of formulation of ZnO nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors: An in vitro study on human skin cells exposed to UVA radiation.

Application of ZnO nanoparticles in sunscreens exposes human skin with their adverse effects, which correlates to dissolution/translocation of free Zn ions. The possibility of decreasing solubility and therefore, reducing toxicity, by structural modifications have been discussed as a solution. The present investigation has developed new metallic lattices of ZnO to reduce cytotoxicity of ZnO nanoparticles. Novel metal-promoted Zn-based nanocomposites ([Zn(O)/M], M = Mg, Al, Ca, Ti) were synthesized and their physicochemical properties and their cytotoxicity were evaluated. Solubility and release studies showed that modification of ZnO structure decreases release of Zn into culture medium. XRD and UV absorbance analyses showed that metallic-dopants percolate into crystalline lattice of ZnO. This phenomenon is basic reason for stability of Zn-based network. Cell culture studies and MTT assay on human skin cells (HFF-1) exposed to UVA radiation showed that the level of protection of [Zn(O)/M] compounds were more than of [ZnO]. Dichlorofluoroscein diacetate-ROS assay and Zn release experiments indicated that [Zn(O)/M] nanocomposites decreased the level of ROS generation and Zn release in compared to ZnO, indicating higher safety of nanocomposites. This study shows that the synthesized Zn-based nanocomposites have potential to be used as safer and more effective sunscreens than ZnO.