Gaonkar Roopa, Shiralgi Yallappa, Lakkappa Dhananjaya B, Hegde Gurumurthy
Department of Biotechnology, B.M.S College of Engineering, Bangalore, 560019, Karnataka, India.
Centre for Nano-materials & Displays, B.M.S. College of Engineering, Bangalore, 560019, Karnataka, India.
Toxicol Rep. 2018 Apr 5;5:489-496. doi: 10.1016/j.toxrep.2018.03.014. eCollection 2018.
The essential oil of or lemongrass oil (LO) is reported to have antibacterial, antifungal and anticancerous effects. HSP90 is one of the major chaperones responsible for the proper folding of cancer proteins. In this paper we show that the essential oil of significantly suppresses the HSP90 gene expression. The cytotoxicity of the compounds was tested by MTT assay and the gene expression studies were carried out using HEK-293 and MCF-7 cells. Also we tested the efficacy of the major component of this essential oil viz. citral and geraniol in inhibiting the HSP90 expression. The oil was found to be more cytotoxic to MCF-7 cells with different IC values for the oil (69.33 μg/mL), citral (140.7 μg/mL) and geraniol (117 μg/mL). The fold change of expression was calculated by RT-qPCR using ΔΔCt (2) method and it was 0.1 and 0.03 in MCF-7 cells at 80 μg/mL and 160 μg/mL of LO. Western blot results showed suppression of HSP90 protein expression and HSP90 - ATPase activity inhibition was also observed using LO. This study shows the anticancer mechanism exhibited by the essential oil of is by the inhibition of the important chaperone protein HSP90.
柠檬草油（LO）据报道具有抗菌、抗真菌和抗癌作用。热休克蛋白90（HSP90）是负责癌症蛋白正确折叠的主要伴侣蛋白之一。在本文中，我们表明柠檬草油能显著抑制HSP90基因表达。通过MTT法检测化合物的细胞毒性，并使用HEK - 293和MCF - 7细胞进行基因表达研究。我们还测试了这种精油的主要成分柠檬醛和香叶醇抑制HSP90表达的功效。发现该油对MCF - 7细胞的细胞毒性更大，油（69.33μg/mL）、柠檬醛（140.7μg/mL）和香叶醇（117μg/mL）的IC值不同。使用ΔΔCt（2）方法通过RT - qPCR计算表达倍数变化，在80μg/mL和160μg/mL的柠檬草油作用下，MCF - 7细胞中的表达倍数变化分别为0.1和0.03。蛋白质印迹结果显示HSP90蛋白表达受到抑制，使用柠檬草油还观察到HSP90 - ATP酶活性受到抑制。这项研究表明柠檬草油展现出的抗癌机制是通过抑制重要的伴侣蛋白HSP90。
