Dunnett Stephen B, Brooks Simon P
Brain Repair Group, Cardiff University, Cardiff, UK.
The Brain Repair Group, School of Biosciences, Cardiff University, Cardiff, UK.
Methods Mol Biol. 2018;1780:121-141. doi: 10.1007/978-1-4939-7825-0_7.
Motor deficits are a characteristic consequence of striatal damage, whether induced by experimental lesions, or in genetic models of Huntington's disease involving polyglutamine expansion in the huntingtin protein. With the growing power of genetic models and genetic tools for analysis, mice are increasingly the animal model of choice, and objective quantitative measures of motor performance are in demand for experimental analysis of disease pathophysiology, progression, and treatment. We present methodological protocols for six of the most common tests of motor function-ranging from spontaneous activity, locomotor coordination, balance, and skilled limb use-that are simple, effective, efficient, and widely used for motor assessment in Huntington's disease research in experimental mice.
运动功能障碍是纹状体损伤的典型后果，无论是由实验性损伤引起，还是在涉及亨廷顿蛋白中多聚谷氨酰胺扩展的亨廷顿病遗传模型中。随着遗传模型和遗传分析工具的能力不断增强，小鼠越来越成为首选的动物模型，并且对于疾病病理生理学、进展和治疗的实验分析，需要客观的运动表现定量测量方法。我们提出了六种最常见的运动功能测试方法的方案，这些测试涵盖自发活动、运动协调、平衡和熟练肢体使用，它们简单、有效、高效，并且在实验小鼠的亨廷顿病研究中广泛用于运动评估。
