拓扑异构酶II是有丝分裂染色体支架的结构成分。
Topoisomerase II is a structural component of mitotic chromosome scaffolds.
作者信息
Earnshaw W C, Halligan B, Cooke C A, Heck M M, Liu L F
出版信息
J Cell Biol. 1985 May;100(5):1706-15. doi: 10.1083/jcb.100.5.1706.
Abstract
We have obtained a polyclonal antibody that recognizes a major polypeptide component of chicken mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr 170,000. Indirect immunofluorescence and subcellular fractionation experiments confirm that it is present in both mitotic chromosomes and interphase nuclei. Two lines of evidence suggest that this protein is DNA topoisomerase II, an abundant nuclear enzyme that controls DNA topological states: anti-scaffold antibody inhibits the strand-passing activity of DNA topoisomerase II; and both anti-scaffold antibody and an independent antibody raised against purified bovine topoisomerase II recognize identical partial proteolysis fragments of the 170,000-mol-wt scaffold protein in immunoblots. Our results suggest that topoisomerase II may be an enzyme that is also a structural protein of interphase nuclei and mitotic chromosomes.
摘要
我们获得了一种多克隆抗体，它能识别鸡有丝分裂染色体支架的一种主要多肽成分。这种多肽在SDS-PAGE中的迁移率对应分子量为170,000。间接免疫荧光和亚细胞分级分离实验证实，它存在于有丝分裂染色体和间期细胞核中。有两条证据表明这种蛋白质是DNA拓扑异构酶II，一种控制DNA拓扑状态的丰富核酶：抗支架抗体抑制DNA拓扑异构酶II的链通过活性；抗支架抗体和针对纯化的牛拓扑异构酶II产生的一种独立抗体在免疫印迹中识别170,000道尔顿支架蛋白相同的部分蛋白水解片段。我们的结果表明，拓扑异构酶II可能是一种既是间期细胞核又是有丝分裂染色体结构蛋白的酶。
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