Labhart P, Koller T, Wunderli H
Cell. 1982 Aug;30(1):115-21. doi: 10.1016/0092-8674(82)90017-4.
Using electron microscopy we show that the metaphase chromatin fibers of Chinese hamster ovary cells form the same ionic strength-dependent higher order structures as the corresponding interphase chromatin fibers. We present evidence that such intact chromatin fibers are a prerequisite for the maintenance of the characteristic shape of metaphase chromosomes. The evidence is based on the finding that treatment of chromosomes with 0.5 M NaCl, a condition which is known to remove histone H1 and which destroys the higher order structure of chromatin fibers, also leads to a disintegration of the metaphase chromosome structure, whereas treatment with 0.3 M (or less) NaCl has no effect on the integrity of the chromosomes and their chromatin fibers. These data support a model in which the metaphase chromosome is maintained by a tight assembly of the 25-30 nm thick chromatin fibers containing all the histones.
利用电子显微镜，我们发现中国仓鼠卵巢细胞的中期染色质纤维形成了与相应间期染色质纤维相同的离子强度依赖性高阶结构。我们提供的证据表明，这种完整的染色质纤维是维持中期染色体特征形状的先决条件。该证据基于以下发现：用0.5M NaCl处理染色体，已知这种条件会去除组蛋白H1并破坏染色质纤维的高阶结构，这也会导致中期染色体结构解体，而用0.3M（或更低）NaCl处理对染色体及其染色质纤维的完整性没有影响。这些数据支持了一个模型，其中中期染色体是由包含所有组蛋白的25 - 30nm厚的染色质纤维紧密组装维持的。
