Berrios M, Osheroff N, Fisher P A
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4142-6. doi: 10.1073/pnas.82.12.4142.
DNA topoisomerase II has been immunochemically identified on protein blots as a major polypeptide component of the Drosophila nuclear matrix-pore complex-lamina fraction. Indirect immunofluorescence analyses of larval cryosections have confirmed the nuclear localization of topoisomerase II in situ. Although apparently excluded from the nucleolus, the topoisomerase protein is otherwise distributed throughout the interior of interphase nuclei. Similar immunocytochemical studies performed with permeabilized whole giant cells from third-instar larval salivary glands have shown topoisomerase II to be largely restricted to the polytene chromosomes. Upon nuclear disassembly during mitosis, the topoisomerase polypeptide appears to redistribute diffusely throughout the cell. Faint immunofluorescent staining of mitotic chromosomes is also observed.
DNA拓扑异构酶II已在蛋白质印迹上通过免疫化学鉴定为果蝇核基质-核孔复合体-核纤层组分的主要多肽成分。幼虫冷冻切片的间接免疫荧光分析已在原位证实拓扑异构酶II的核定位。尽管拓扑异构酶蛋白显然被排除在核仁之外，但它在其他方面分布于间期细胞核的内部。对三龄幼虫唾液腺的通透化全巨细胞进行的类似免疫细胞化学研究表明，拓扑异构酶II主要局限于多线染色体。在有丝分裂期间核解体时，拓扑异构酶多肽似乎在整个细胞中弥散重新分布。还观察到有丝分裂染色体的微弱免疫荧光染色。
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