Collins D M, De Lisle G W
J Clin Microbiol. 1985 Apr;21(4):562-4. doi: 10.1128/jcm.21.4.562-564.1985.
DNA preparations from 24 New Zealand isolates, two reference strains of Mycobacterium bovis, and one reference strain each of Mycobacterium microti, Mycobacterium africanum, and Mycobacterium tuberculosis were characterized by restriction endonuclease analysis. Twenty-five restriction enzymes were investigated. The clearest differences in M. bovis patterns were obtained with the enzymes BstEII and BclI. These produced four and five different patterns, respectively, for the 24 local isolates. When the results from both enzymes were considered, seven different combinations were obtained. The patterns produced for the two reference strains of M. bovis could be distinguished from each other and also from the patterns produced for the local isolates. All patterns were reproducible and are now being used for typing M. bovis isolates. With either enzyme, the patterns produced for the M. tuberculosis, M. bovis, and M. africanum strains had many features in common, but all the M. bovis patterns were clearly more similar to each other than to the M. tuberculosis patterns. The patterns produced for the M. microti strain were markedly different from those produced for the other species. Restriction endonuclease analysis is clearly a useful method for inter- and intraspecific classifications of the tuberculosis complex.
通过限制性内切酶分析对来自24株新西兰分离株、两株牛分枝杆菌参考菌株以及微小分枝杆菌、非洲分枝杆菌和结核分枝杆菌各一株参考菌株的DNA制剂进行了特征分析。研究了25种限制性酶。用BstEII和BclI酶获得了牛分枝杆菌模式中最明显的差异。这两种酶分别为24株本地分离株产生了四种和五种不同的模式。当考虑两种酶的结果时，获得了七种不同的组合。牛分枝杆菌的两株参考菌株产生的模式彼此可以区分，也与本地分离株产生的模式不同。所有模式均可重复，目前正用于牛分枝杆菌分离株的分型。使用任何一种酶，结核分枝杆菌、牛分枝杆菌和非洲分枝杆菌菌株产生的模式有许多共同特征，但所有牛分枝杆菌模式彼此之间明显比与结核分枝杆菌模式更相似。微小分枝杆菌菌株产生的模式与其他物种产生的模式明显不同。限制性内切酶分析显然是用于结核分枝杆菌复合群种间和种内分类的一种有用方法。
