Barrow P A
J Hyg (Lond). 1981 Jun;86(3):247-57. doi: 10.1017/s0022172400068996.
A relatively simple method for the detection of intra-specific variation of Mycobacterium bovis is described, based on the ability of strains to utilize or remove amino acids from solution. Using the method, 31 strains of M. bovis, isolated badgers and cattle, have been divided in to four major groups with some additional sub-groups and intermediate strains. Preliminary results suggest that the biotypes observed are relatively stable. The method was used in the investigation of an outbreak of bovine tuberculosis in cattle in Gloucestershire where badgers were implicated as a source of infection. The results suggested that transmission of infection had occurred on at least two or three separate occasions. It is suggested that the method could be used in studying the epidemiology of bovine tuberculosis in wild animals, cattle and man.
本文描述了一种相对简单的检测牛分枝杆菌种内变异的方法，该方法基于菌株从溶液中利用或去除氨基酸的能力。利用该方法，从獾和牛中分离出的31株牛分枝杆菌被分为四个主要组，还有一些额外的亚组和中间菌株。初步结果表明所观察到的生物型相对稳定。该方法被用于调查格洛斯特郡牛群中牛结核病的爆发，此次疫情中獾被认为是感染源。结果表明感染传播至少发生在两到三个不同的场合。建议该方法可用于研究野生动物、牛和人类中牛结核病的流行病学。
