Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada.
Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
J Periodontol. 2018 May;89(5):586-595. doi: 10.1002/JPER.17-0352.
Products of internal defense systems, like pro-inflammatory cytokines, reactive oxygen species, and leukocytes, are released which attack periodontal bacteria in periodontitis, but at the same time, lead to tissue destruction as well. We hypothesize that resveratrol derivative-rich melinjo seed extract (MSE), an edible plant extract that has antioxidant properties, should promote healing of periodontal bone loss and modulating immune-inflammatory systems that leads periodontal tissue destruction.
We used an experimentally induced periodontitis (EP) model in mice. Ligatures were placed first for development of EP (15 days). MSE was intraperitoneally administrated (0.001% (w/w)) to reverse bone loss that had already occurred in established EP and mice were then sacrificed (day 17, 20 and 22).
Morphometric outcomes revealed lower bone-loss in the MSE groups compared to control. Immunohistochemistry assays demonstrated lower oxidative stress in MSE groups. MSE also inhibited M-CSF/sRANKL mediated osteoclast formation and down-regulated osteoclast activity.
Treatment with MSE in EP actually caused healing of bone, and these effects are probably related to decreases in local oxidative damage and osteoclast activity. Given MSE's positive effects on osteodifferentiation as well, these findings suggest that MSE could be a useful therapeutic agent for the management of periodontitis.
内防御系统的产物，如促炎细胞因子、活性氧物质和白细胞，被释放出来，攻击牙周炎中的牙周细菌，但同时也导致组织破坏。我们假设富含白藜芦醇衍生物的 Melinjo 种仁提取物（MSE），一种具有抗氧化特性的食用植物提取物，应该能促进牙周骨丧失的愈合，并调节导致牙周组织破坏的免疫炎症系统。
我们使用了一种实验性诱导的牙周炎（EP）小鼠模型。首先结扎以诱导 EP 的发展（15 天）。MSE 通过腹腔内给药（0.001%（w/w））以逆转已经发生在既定 EP 中的骨丢失，然后处死小鼠（第 17、20 和 22 天）。
形态计量学结果显示 MSE 组的骨丢失较低。免疫组织化学检测显示 MSE 组的氧化应激较低。MSE 还抑制了 M-CSF/sRANKL 介导的破骨细胞形成，并下调了破骨细胞活性。
EP 中 MSE 的治疗实际上导致了骨的愈合，这些效果可能与局部氧化损伤和破骨细胞活性的降低有关。鉴于 MSE 对成骨分化也有积极的影响，这些发现表明 MSE 可能是治疗牙周炎的一种有用的治疗剂。
