单体和二聚体白藜芦醇在小鼠牙周炎模型中的治疗效果。
Healing effects of monomer and dimer resveratrol in a mouse periodontitis model.
机构信息
Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, 113-8549, Bunkyo, Tokyo, Japan.
Department of Molecular Immunology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, 113-8549, Bunkyo, Tokyo, Japan.
出版信息
BMC Oral Health. 2022 Nov 1;22(1):460. doi: 10.1186/s12903-022-02499-2.
BACKGROUND
The antioxidant and anti-inflammatory effects of resveratrol have been reported previously. Particularly, monomeric trans-resveratrol has been demonstrated to produce positive effects in various pathological processes. We reported previously that resveratrol dimer-rich melinjo extract, among others, caused bone healing, decreased local oxidative damage, and activated antioxidants nuclear factor erythroid 2-related factor 2 (Nrf2) pathways in a mouse model of experimentally induced periodontitis (EP). This study aimed to compare the bone-healing effects of the resveratrol monomer to the resveratrol dimer (gnetin C found in melinjo seed extract) in a model of EP and investigate the involvement of Nrf2 for effects of either form of resveratrol.
METHODS
EP was induced experimentally in mice by placement of a 9 - 0 silk ligature around the left second molar. Mice received 10 mg/kg of either resveratrol monomer or dimer intraperitoneally on day 15 after induction of EP. The bone level around the ligated teeth was measured over time, and levels of proinflammatory cytokines and oxidative stress were measured in the periodontal tissues around the ligated teeth.
RESULTS
Resveratrol dimer induced greater periodontal bone healing as compared to that related to use of the resveratrol monomer. It appears that healing of periodontal bone in either group was likely related to master regulation of antioxidant nuclear factor erythroid 2-related factor 2 (Nrf2) significantly. Downregulation of IL-1β, a proinflammatory cytokine was also demonstrated in the resveratrol dimer group.
CONCLUSION
Our results showed that administration of resveratrol in either dimer form or the monomeric form reduced periodontal bone loss with greater inhibition of bone loss being demonstrated in the dimer group as compared to the monomer group and that these effects were related in all likelihood to decreased oxidative stress and hence reduction in local inflammation.
背景
白藜芦醇具有抗氧化和抗炎作用，此前已有报道。特别是单体反式白藜芦醇已被证明在各种病理过程中产生积极影响。我们之前曾报道过，丰富白藜芦醇二聚体的 Melinjo 提取物等可促进骨愈合，减少局部氧化损伤，并激活核因子红细胞 2 相关因子 2 (Nrf2) 途径，这在实验性牙周炎 (EP) 小鼠模型中得到了证实。本研究旨在比较单体白藜芦醇和二聚体（在 Melinjo 种子提取物中发现的 Gnetin C）在 EP 模型中的骨愈合作用，并研究 Nrf2 对这两种形式的白藜芦醇作用的影响。
方法
通过在左侧第二磨牙周围放置 9-0 丝线结扎来在小鼠中诱导 EP。在 EP 诱导后 15 天，小鼠每天腹膜内注射 10mg/kg 的单体或二聚体白藜芦醇。随着时间的推移测量结扎牙周围的骨水平，并测量结扎牙周围牙周组织中的促炎细胞因子和氧化应激水平。
结果
与单体白藜芦醇相比，二聚体白藜芦醇诱导了更大的牙周骨愈合。两种白藜芦醇形式都可能与抗氧化核因子红细胞 2 相关因子 2 (Nrf2) 的主调控有关。促炎细胞因子 IL-1β 的下调也在二聚体组中得到证实。
结论
我们的研究结果表明，无论是二聚体形式还是单体形式的白藜芦醇给药均可减少牙周骨丢失，二聚体组的骨丢失抑制作用大于单体组，这些作用很可能与氧化应激减少有关，从而减少局部炎症。
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