Contreras Lpc, Dal Piva Amo, Ribeiro F C, Anami L C, Camargo Sea, Jorge Aoc, Bottino M A
Oper Dent. 2018 Nov/Dec;43(6):593-601. doi: 10.2341/17-126-L. Epub 2018 Jun 1.
: Feldspathic ceramic restorations can be obtained by different techniques (stratification or computer-aided design/computer-aided manufacturing [CAD/CAM] blocks) and finishing procedures (polishing or glaze application). This study evaluated the effects of techniques and finishing procedures on surface properties, biofilm formation, and viability of human gingival fibroblasts (FMM-1) in contact with these materials.
: Ceramic specimens were obtained through a stratification technique (Vita VM9) and from CAD/CAM blocks (Vita Blocs Mark II; both Vita Zahnfabrik) and their surfaces were finished by polishing (ceramisté diamond rubbers + polishing paste; "p" subgroups) or glaze spray application + sintering ("g" subgroups). Roughness (Ra and RSm parameters) and surface free energy (SFE) were measured. Early biofilm formation of Streptococcus mutans, Streptococcus sanguinis, and Candida albicans was evaluated by counting colony-forming units (CFU). MTT (3-[4.5-dimethyl-thiazol-2-yl-]-2.5-diphenyl tetrazolium bromide) cytotoxicity test evaluated cellular viability for the growth of FMM-1 after 24 hours and seven days of contact. Scanning electron microscopy (SEM) and three-dimensional optical profilometry were performed to qualitatively analyze the surface. Data were analyzed by analysis of variance, Tukey test, and t-test (all α=0.05).
: Polished samples presented lower roughness (Ra, p=0.015; RSm, p=0.049) and higher SFE ( p=0.00). Streptococci had higher CFU in all groups, but the CFU of C albicans was lower for polished samples. Biofilm formation was influenced by the interaction of all factors ( p=0.018), and the materials showed no cytotoxicity to FMM-1 growth.
: Polishing resulted in the lowest values for surface roughness and higher SFE values. Polished ceramics showed less C albicans adherence while the adherence of Streptococci was greater than C albicans in all conditions.
长石质陶瓷修复体可通过不同技术（分层或计算机辅助设计/计算机辅助制造[CAD/CAM]块）和精加工程序（抛光或施釉）获得。本研究评估了技术和精加工程序对与这些材料接触的人牙龈成纤维细胞（FMM-1）的表面特性、生物膜形成和活力的影响。
通过分层技术（Vita VM9）和从CAD/CAM块（Vita Blocs Mark II；均为Vita Zahnfabrik）获得陶瓷标本，并通过抛光（陶瓷师金刚石橡胶+抛光膏；“p”亚组）或喷雾施釉+烧结（“g”亚组）对其表面进行精加工。测量粗糙度（Ra和RSm参数）和表面自由能（SFE）。通过计数菌落形成单位（CFU）评估变形链球菌、血链球菌和白色念珠菌的早期生物膜形成。MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑）细胞毒性试验评估接触24小时和7天后FMM-1生长的细胞活力。进行扫描电子显微镜（SEM）和三维光学轮廓测量以定性分析表面。数据通过方差分析、Tukey检验和t检验进行分析（所有α=0.05）。
抛光样品的粗糙度较低（Ra，p=0.015；RSm，p=0.049），表面自由能较高（p=0.00）。所有组中链球菌的CFU均较高，但抛光样品中白色念珠菌的CFU较低。生物膜形成受所有因素相互作用的影响（p=0.018），且材料对FMM-1生长无细胞毒性。
抛光导致表面粗糙度值最低和表面自由能值较高。抛光陶瓷显示白色念珠菌的粘附较少，而在所有条件下链球菌的粘附均大于白色念珠菌。
