a School of Psychology and Exercise Science, Murdoch University, Murdoch 6150, Western Australia, Australia.
b School of Health Sciences, Murdoch University, Western Australia, Fremantle 6160, Australia.
Appl Physiol Nutr Metab. 2018 Dec;43(12):1298-1306. doi: 10.1139/apnm-2018-0160. Epub 2018 Jun 1.
This study examined the effect of 2 forms of exercise on glucose tolerance and the concurrent changes in markers associated with the interleukin (IL)-6 pathways. Fifteen sedentary, overweight males (29.0 ± 3.1 kg/m) completed 2 separate, 3-day trials in randomised and counterbalanced order. An oral glucose tolerance test (OGTT; 75 g) was performed at the same time on each day of the trial. Day 2 of each trial consisted of a single 30-min workload-matched bout of either high-intensity intermittent exercise (HIIE; alternating 100% and 50% of peak oxygen uptake) or continuous moderate-intensity exercise (CME; 60 % of peak oxygen uptake) completed 1 h prior to the OGTT. Venous blood samples were collected before, immediately after, 1 h after, and 25 h after exercise for measurement of insulin, C-peptide, IL-6, and the soluble IL-6 receptors (sIL-6R; soluble glycoprotein 130 (sgp130)). Glucose area under the curve (AUC) was calculated from capillary blood samples collected throughout the OGTT. Exercise resulted in a modest (4.4%; p = 0.003) decrease in the glucose AUC when compared with the pre-exercise AUC; however, no differences were observed between exercise conditions (p = 0.65). IL-6 was elevated immediately after and 1 h after exercise, whilst sgp130 and sIL-6R concentrations were reduced immediately after exercise. In summary, exercise was effective in reducing glucose AUC, which was attributed to improvements that took place between 60 and 120 min into the OGTT, and was in parallel with an increased ratio of IL-6 to sIL-6R, which accords with an increased activation via the "classical" IL-6 signalling pathway. Our findings suggest that acute HIIE did not improve glycaemic response when compared with CME.
这项研究考察了两种运动形式对葡萄糖耐量的影响，以及与白细胞介素 (IL)-6 通路相关的标志物的变化。15 名久坐、超重的男性（29.0±3.1kg/m）以随机和平衡的顺序完成了 2 项单独的 3 天试验。在试验的每一天，都进行了口服葡萄糖耐量试验 (OGTT；75g)。每次试验的第 2 天，在 OGTT 前 1 小时，进行一次单次 30 分钟的工作负荷匹配的高强度间歇运动（HIIE；交替 100%和 50%的峰值摄氧量）或连续中等强度运动（CME；60%的峰值摄氧量）。在运动前、运动后立即、运动后 1 小时和运动后 25 小时采集静脉血样，用于测量胰岛素、C 肽、IL-6 和可溶性白细胞介素 6 受体（sIL-6R；可溶性糖蛋白 130 (sgp130)）。通过在 OGTT 期间采集的毛细血管血样计算葡萄糖 AUC。与运动前 AUC 相比，运动导致葡萄糖 AUC 适度（4.4%；p=0.003）降低；然而，两种运动条件之间没有观察到差异（p=0.65）。IL-6 在运动后立即和 1 小时后升高，而 sgp130 和 sIL-6R 浓度在运动后立即降低。总之，运动有效降低了葡萄糖 AUC，这归因于 OGTT 进行到 60-120 分钟时发生的改善，并且与 IL-6 与 sIL-6R 的比值增加平行，这与通过“经典”IL-6 信号通路的增加激活一致。我们的研究结果表明，与 CME 相比，急性 HIIE 并没有改善血糖反应。
