Boanini Elisa, Cassani Maria Cristina, Rubini Katia, Boga Carla, Bigi Adriana
Department of Chemistry "Giacomo Ciamician", University of Bologna, via Selmi, 2, 40126 Bologna, Italy.
Department of Industrial Chemistry "Toso Montanari", Via del Risorgimento, 4, 40136 Bologna, Italy.
Nanomaterials (Basel). 2018 May 31;8(6):390. doi: 10.3390/nano8060390.
Functionalization of calcium phosphates for biomedical applications has been proposed as a strategy to enrich the good osteoinductive properties of these materials with specific therapeutic characteristics. Herein, we prepared and characterized hydroxyapatite nanocrystals functionalized with an anticancer agent, ()-9-hydroxystearate (HSA), and loaded with an antimicrobial agent, namely silver nanoparticles (AgNPs). Nanocrystals at two different contents of HSA, about 4 and 9 wt %, were prepared via direct synthesis in aqueous solution. Loading with the antibacterial agent was achieved through interaction with different volumes of AgNPs suspensions. The amount of loaded nanoparticles increases with the volume of the AgNPs suspension and with the hydroxystearate content of the nanocrystals, up to about 3.3 wt %. The structural, morphological, and hydrophobic properties of the composite materials depend on hydroxystearate content, whereas they are not affected by AgNPs loading. At variance, the values of zeta potential slightly increase with the content of AgNPs, which exhibit a sustained release in cell culture medium.
磷酸钙的功能化已被提议作为一种策略，通过特定的治疗特性来丰富这些材料良好的骨诱导性能。在此，我们制备并表征了用抗癌剂（）-9-羟基硬脂酸（HSA）功能化并负载抗菌剂即银纳米颗粒（AgNPs）的羟基磷灰石纳米晶体。通过在水溶液中直接合成制备了两种不同HSA含量（约4 wt%和9 wt%）的纳米晶体。通过与不同体积的AgNPs悬浮液相互作用实现抗菌剂的负载。负载的纳米颗粒数量随着AgNPs悬浮液的体积以及纳米晶体中羟基硬脂酸含量的增加而增加，最高可达约3.3 wt%。复合材料的结构、形态和疏水性能取决于羟基硬脂酸含量，而不受AgNPs负载的影响。与此不同的是，ζ电位值随着AgNPs含量的增加而略有增加，AgNPs在细胞培养基中呈现持续释放。
