Lijnen P, M'Buyamba-Kabangu J R, Lissens W, Amery A
Methods Find Exp Clin Pharmacol. 1985 Jan;7(1):35-40.
The erythrocyte concentrations and the transmembrane fluxes of sodium and potassium were investigated in normal men and also in twenty normal women during the two stages of the menstrual cycle. Half of them were taking the contraceptive pill and the other half were not. In women with a normal menstrual cycle, the erythrocyte sodium concentration (Naic) and the ouabain-insensitive total potassium efflux were lower in the luteal than in the follicular phase. Intracellular potassium concentration (Kic), ouabain-sensitive 86rubidium-uptake and the furosemide-sensitive Na+ and K+ efflux did not differ significantly between the two periods of the cycle. No cycle-related variation in Naic or Kic was observed in women using the contraceptive pill. In these women, however, the ouabain-sensitive 86rubidium-uptake was increased in the second part of the menstrual cycle. Compared to men, the intra-erythrocyte sodium concentration was lower in women during the second stage of the menstrual cycle. These two groups were similar for Na+, K+-ATPase pump activity estimated from the ouabain-sensitive 86rubidium-uptake and for the furosemide-sensitive sodium and potassium efflux. Women in the first stage of the menstrual cycle had intra-erythrocyte sodium concentration similar to men, but their furosemide-sensitive sodium efflux was lower. No significant difference was observed in the intraerythrocyte potassium concentration and transmembrane fluxes of potassium in men and women in either stage of the menstrual cycle. We therefore conclude from this study that one should take into account sex-related variability when studying cationic fluxes and concentrations in red blood cells of men and women.
在正常男性以及20名正常女性的月经周期两个阶段，对红细胞中钠和钾的浓度以及跨膜通量进行了研究。其中一半女性服用避孕药，另一半未服用。在月经周期正常的女性中，黄体期的红细胞钠浓度（Naic）和哇巴因不敏感的总钾外流低于卵泡期。细胞内钾浓度（Kic）、哇巴因敏感的86铷摄取以及速尿敏感的钠和钾外流在月经周期的两个阶段之间没有显著差异。服用避孕药的女性未观察到Naic或Kic的周期相关变化。然而，在这些女性中，哇巴因敏感的86铷摄取在月经周期的第二阶段增加。与男性相比，月经周期第二阶段女性的红细胞内钠浓度较低。从哇巴因敏感的86铷摄取估计的Na +、K + -ATP酶泵活性以及速尿敏感的钠和钾外流在这两组中相似。月经周期第一阶段的女性红细胞内钠浓度与男性相似，但她们速尿敏感的钠外流较低。在月经周期的任何阶段，男性和女性的红细胞内钾浓度和钾的跨膜通量均未观察到显著差异。因此，我们从这项研究得出结论，在研究男性和女性红细胞中的阳离子通量和浓度时，应考虑性别相关的变异性。
