Li Yi-Fan, Li Tie, Zhang Da-Wei, Xue Hui, Chen Dong, Li Chen, Wang Fu-Chun
Department of Anatomy, School of Basic Medical Science, Changchun University of Chinese Medicine, Changchun 130117, China.
Department of Acupuncture, School of Acupuncture and Massage, Changchun University of Chinese Medicine, Changchun 130117, China.
Evid Based Complement Alternat Med. 2018 May 9;2018:7568697. doi: 10.1155/2018/7568697. eCollection 2018.
The present study aimed to evaluate the role of the combination treatment of methylprednisolone (MP) and electroacupuncture (EA) in regeneration of nerve fibers and functional recovery in rats with spinal cord injury (SCI). Female Wistar rats were used for an SCI model by using a weight-drop hammer at levels T (spinal cord segment corresponding to the 10th thoracic vertebra). Four groups received different treatments for the study: SCI control, MP, MP and EA, and Sham. The growth of nerve fibers was examined by counting fluorescein positive nerve fibers. The motor functional recovery was evaluated by Basso, Beattie, Bresnahan (BBB) score, and electrophysiology analysis. We found that, compared to MP groups, there were more well-oriented and paralleled fluorescein positive nerve fibers in MP and EA group. Both latencies and amplitudes of the Motor Evoked Potential (MEP) in the combination therapy of MP and EA were higher than MP group. Additionally, recovered hindlimb movements were sustained in most rats in the MP and EA group. Our study indicated that combination therapies could become a powerful treatment for SCI in rats.
本研究旨在评估甲基强的松龙（MP）与电针（EA）联合治疗对脊髓损伤（SCI）大鼠神经纤维再生和功能恢复的作用。采用体重下落锤在T水平（对应第10胸椎的脊髓节段）建立雌性Wistar大鼠SCI模型。四组接受不同治疗用于该研究：SCI对照组、MP组、MP与EA联合组以及假手术组。通过计数荧光素阳性神经纤维来检测神经纤维的生长情况。通过Basso、Beattie、Bresnahan（BBB）评分和电生理分析来评估运动功能恢复情况。我们发现，与MP组相比，MP与EA联合组中有更多排列良好且平行的荧光素阳性神经纤维。MP与EA联合治疗组的运动诱发电位（MEP）的潜伏期和波幅均高于MP组。此外，MP与EA联合组的大多数大鼠后肢运动恢复得以维持。我们的研究表明联合治疗可能成为治疗大鼠SCI的有效方法。
Zotero 插件
