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一种用于即时光学检测人肺泡肺组织中活细菌的标记泛杀素抗菌肽。

A labelled-ubiquicidin antimicrobial peptide for immediate optical detection of live bacteria in human alveolar lung tissue.

作者信息

Akram Ahsan R, Avlonitis Nicolaos, Lilienkampf Annamaria, Perez-Lopez Ana M, McDonald Neil, Chankeshwara Sunay V, Scholefield Emma, Haslett Christopher, Bradley Mark, Dhaliwal Kevin

机构信息

Pulmonary Optical Molecular Imaging Group , MRC Centre for Inflammation Research , Queen's Medical Research Institute , 47 Little France Crescent , Edinburgh , EH16 4TJ , UK . Email:

EaStCHEM , The University of Edinburgh School of Chemistry , Joseph Black Building, West Mains Road , Edinburgh EH9 3FJ , UK . Email:

出版信息

Chem Sci. 2015 Dec 1;6(12):6971-6979. doi: 10.1039/c5sc00960j. Epub 2015 Jun 29.

DOI:10.1039/c5sc00960j
PMID:29861935
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5947527/
Abstract

The immediate detection of the presence of bacteria in the distal human lung is of significant clinical utility. Herein we describe the development and optimization of a bacterial binding fragment (UBI) of the antimicrobial peptide, ubiquicidin (UBI), conjugated to an environmentally sensitive fluorophore to enable rapid live bacterial imaging within human lung tissue. UBI was modified for stability in the presence of human lung bronchoalveolar lavage fluid, for affinity to bacterial membranes and functionality in human lung tissue. The optimized cyclic structure yields an optical molecular Smartprobe for bacterial detection in human lung tissue.

摘要

在人类肺远端即时检测细菌的存在具有重要的临床实用价值。在此，我们描述了抗菌肽泛菌素（UBI）的细菌结合片段（UBI）的开发和优化，该片段与一种对环境敏感的荧光团偶联，以便在人类肺组织内进行快速的活细菌成像。对UBI进行了修饰，使其在人类肺支气管肺泡灌洗液存在的情况下具有稳定性，对细菌膜具有亲和力，并在人类肺组织中具有功能。优化后的环状结构产生了一种用于人类肺组织细菌检测的光学分子智能探针。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/d0eadc42eba1/c5sc00960j-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/a16e28e12892/c5sc00960j-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/cef095b6dc2a/c5sc00960j-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/aa41082ca91f/c5sc00960j-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/d0eadc42eba1/c5sc00960j-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/a16e28e12892/c5sc00960j-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/f6ba54d5c3b2/c5sc00960j-f2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/ac3784477e03/c5sc00960j-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/64b20ea343b3/c5sc00960j-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/2f5d4e78315a/c5sc00960j-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/9359bfe49d05/c5sc00960j-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/cef095b6dc2a/c5sc00960j-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/aa41082ca91f/c5sc00960j-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b11/5947527/d0eadc42eba1/c5sc00960j-f10.jpg

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