早产儿视网膜病变中差异表达的微小RNA——一项生物信息学分析
Differentially expressed miRNAs in premature infants with retinopathy-a bioinformatics analysis.
作者信息
Yang Yang, Pan Jing-Jing, Zhou Xiao-Guang, Zhou Xiao-Yu, Cheng Rui
机构信息
Department of Neonatology, Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.
Department of Neonatology, Jiangsu Provincial People's Hospital Affiliated to Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
出版信息
Int J Ophthalmol. 2018 May 18;11(5):773-779. doi: 10.18240/ijo.2018.05.09. eCollection 2018.
AIM
To reveal the role of miRNAs in retinopathy of prematurity (ROP) by bioinformatics analysis.
METHODS
The raw data of this study came from the researches of Wang and Zhao who analyzed the microRNA (miRNA) expression profile between ROP and controls. Based on the identified differentially expressed miRNAs, the related target genes, lncRNA and circRNA were predicted. Then we performed functional enrichment analysis to further analyze the functions of target genes.
RESULTS
Hsa-miRNA-128-3p and hsa-miRNA-9-5p showed significantly different expression in both studies. LncRNA of POLDIP2, GAS5, NEFL and UHRF1, circRNA of ZNF280C_hsa_circ_001211 and SIAE_hsa_circ_002083, tar-get gene of QKI showed meaningful differential expression in ROP. Enrichment analysis showed that TGF-β signaling pathway, PI3K-Akt signaling pathway and MAPK signaling pathway might play important roles in the prog-ress of ROP.
CONCLUSION
This research may provide a comprehensive bioinformatics analysis of differentially expressed miRNAs which are possibly involved in ROP.
目的
通过生物信息学分析揭示微小RNA（miRNA）在早产儿视网膜病变（ROP）中的作用。
方法
本研究的原始数据来自王和赵的研究，他们分析了ROP组与对照组之间的微小RNA（miRNA）表达谱。基于鉴定出的差异表达miRNA，预测相关的靶基因、长链非编码RNA（lncRNA）和环状RNA（circRNA）。然后进行功能富集分析以进一步分析靶基因的功能。
结果
在两项研究中，hsa-miRNA-128-3p和hsa-miRNA-9-5p均表现出显著差异表达。POLDIP2、GAS5、NEFL和UHRF1的lncRNA，ZNF280C_hsa_circ_001211和SIAE_hsa_circ_002083的circRNA，QKI的靶基因在ROP中表现出有意义的差异表达。富集分析表明，转化生长因子-β（TGF-β）信号通路、磷脂酰肌醇-3激酶-蛋白激酶B（PI3K-Akt）信号通路和丝裂原活化蛋白激酶（MAPK）信号通路可能在ROP的进展中起重要作用。
结论
本研究可能为可能参与ROP的差异表达miRNA提供全面的生物信息学分析。
Zotero 插件