Cleary M L, Epstein M A, Finerty S, Dorfman R F, Bornkamm G W, Kirkwood J K, Morgan A J, Sklar J
Science. 1985 May 10;228(4700):722-4. doi: 10.1126/science.2986287.
Cotton-top tamarins were inoculated with sufficient Epstein-Barr virus to induce multiple tumors in each animal within 14 to 21 days. The tumors consisted of large-cell lymphomas that contained multiple copies of the Epstein-Barr virus genome and generated Epstein-Barr virus-carrying cell lines showing no detectable consistent chromosomal abnormality. Hybridization of tumor DNA with immunoglobulin gene probes revealed that each lymphoma was oligo- or monoclonal in origin and that individual tumors from the same animal arose from different B-cell clones. Thus the virus induced multiple transformation events in tamarins in vivo to cause malignant tumors resembling the Epstein-Barr virus-associated lymphomas of patients with organ transplants.
给棉顶狨接种足够的爱泼斯坦-巴尔病毒，以便在14至21天内在每只动物体内诱发多个肿瘤。这些肿瘤由大细胞淋巴瘤组成，其中含有多个爱泼斯坦-巴尔病毒基因组拷贝，并产生携带爱泼斯坦-巴尔病毒的细胞系，这些细胞系未显示出可检测到的一致染色体异常。肿瘤DNA与免疫球蛋白基因探针的杂交显示，每个淋巴瘤在起源上是寡克隆或单克隆的，并且同一动物的单个肿瘤来自不同的B细胞克隆。因此，该病毒在棉顶狨体内诱发了多个转化事件，从而导致了类似于器官移植患者中与爱泼斯坦-巴尔病毒相关的淋巴瘤的恶性肿瘤。
