Miller G, Shope T, Coope D, Waters L, Pagano J, Bornkamn G, Henle W
J Exp Med. 1977 Apr 1;145(4):948-67. doi: 10.1084/jem.145.4.948.
6 of 20 cotton-top tamarins (Saguinus oedipus) inoculated with Epstein-Barr virus (EBV) developed diffuse malignant lymphoma resembling reticulum cell or immunoblastic sarcoma of man. Hyperplastic lymphoreticular lesions were induced in three additional animals; in two instances the hyperplastic lesions regressed. Inapparent infection with development of antibody occured in eight animals. In two animals there was no evidence of EBV infection. One animal died in the first week after inoculation of parasitic infection. 10 animals uninoculated or mock-inoculated developed neither lymphoproliferative disease nor antibody. The malignant lymphoma appeared to arise from a cell with an uncleaved vesicular nucleus found in the center of the germinal follicle. The prominent cytologic features of this cell were extensive formation or rough endoplasmic reticulum and elaboration of the cytoplasmic membrane with microvilli. Cell lines derived from these tumors did not have receptors for complement. IgFc, or sheep erythrocytes, and the cell lines adhered to glass and plastic. EB nuclear antigen was found in imprints of two lymph nodes, one with lymphoma and one with hyperplasia. EB virus DNA was detected directly in the tumors of three animals and in cell lines from two lymphomas. Typical herpes virus particles were found in supernatant fluids from cell lines obtained from lymph nodes with tumors and hyperplasia, as well as in lines derived from blood leukocytes of marmosets with inapparent infection. These virus preparations had the biologic property characteristic of EBV, namely, stimulation of cellular DNA synthesis and immortalization of human lymphocytes. The virus derived from two cell lines was neutralized by reference human sera with EBV antibody and not by antibody-negative human sera. The virus derived from the experimental lesions is thus indistinghishable from human EBV. The marmoset has enhanced susceptibility to oncogenesis by EB virus. Among identified factors which may play a role in the heightened tumorigenicity of EB virus in this species are the increased production of virus by transformed cells and the absence of membrane receptors for complement or IgFc on transformed cells.
20只接种了爱泼斯坦-巴尔病毒（EBV）的棉顶狨猴（Saguinus oedipus）中有6只发生了弥漫性恶性淋巴瘤，类似于人类的网状细胞或免疫母细胞肉瘤。另外3只动物出现了增生性淋巴网状病变；其中2例增生性病变消退。8只动物发生了隐性感染并产生了抗体。2只动物没有EBV感染的证据。1只动物在接种后第一周死于寄生虫感染。10只未接种或假接种的动物既未发生淋巴增生性疾病也未产生抗体。恶性淋巴瘤似乎起源于生发滤泡中心发现的具有未裂解泡状核的细胞。该细胞突出的细胞学特征是粗面内质网广泛形成以及带有微绒毛的细胞膜的 elaboration（此处可能有误，原词可能是“elaboration”，意为精心制作、详尽阐述等，结合语境推测可能是“特化”之类的意思）。从这些肿瘤衍生的细胞系没有补体、IgFc或绵羊红细胞的受体，并且这些细胞系能黏附于玻璃和塑料。在两个淋巴结的印片中发现了EB核抗原，一个淋巴结有淋巴瘤，另一个有增生。在3只动物的肿瘤以及来自两个淋巴瘤的细胞系中直接检测到了EB病毒DNA。在从有肿瘤和增生的淋巴结获得的细胞系的上清液中，以及从隐性感染的狨猴血白细胞衍生的细胞系中发现了典型的疱疹病毒颗粒。这些病毒制剂具有EBV的生物学特性，即刺激细胞DNA合成和使人类淋巴细胞永生化。来自两个细胞系的病毒被具有EBV抗体的参考人血清中和，而未被抗体阴性的人血清中和。因此，从实验性病变中衍生的病毒与人类EBV无法区分。棉顶狨猴对EB病毒诱导肿瘤发生的易感性增强。在已确定的可能在该物种中EB病毒致瘤性增强中起作用的因素包括转化细胞病毒产量增加以及转化细胞上缺乏补体或IgFc的膜受体。
