School of Medicine, University of Belgrade, Belgrade 11000, Serbia.
University Children's Hospital, Belgrade 11000, Serbia.
Asian J Androl. 2018 Sep-Oct;20(5):488-492. doi: 10.4103/aja.aja_35_18.
Autologous tissue engineering using biodegradable scaffolds as a carrier is a well-known procedure for penile girth enhancement. We evaluated a group of previously treated patients with the aim to analyze histomorphometric changes after tissue remodeling and to estimate the benefits of repeated procedure. Between February 2012 and December 2016, a group of 21 patients, aged 22-37 (mean 28.0) years, underwent a repeated penile girth enhancement procedure with biodegradable scaffolds. Procedure included insertion of two poly-lactic-co-glycolic acid scaffolds seeded with laboratory-prepared fibroblasts from scrotal tissue specimens. During this procedure, biopsy specimens of tissue formed after the first surgery were taken for microscopic analysis. The mean follow-up was 38 months. Connective tissue with an abundance of connective tissue fibers, small blood vessels, and inflammatory cells were observed in all analyzed surgically removed tissue. Ultrastructural analysis of these tissue samples discovered the presence of large quantities of collagen fibrils running parallel to each other, forming bundles, with a few widely spread fibroblasts. In total, the mean values of flaccid and erect gain in girth after the second surgery were 1.1 ± 0.4 (range: 0.6-1.7) cm and 1.0 ± 0.3 (range: 0.6-1.5) cm, respectively. Microscopic evaluation of newly formed tissue, induced by autologous tissue engineering using biodegradable scaffolds, showed the presence of vascularized loose connective tissue with an abundance of collagen fibers, fibroblasts, and inflammatory cells, indicating active neovascularization and fibrinogenesis. The benefit of the repeated enhancement procedure was statistically significant.
利用可生物降解支架作为载体的自体组织工程是一种众所周知的阴茎增粗方法。我们评估了一组先前接受过治疗的患者，旨在分析组织重塑后的组织形态计量学变化，并评估重复手术的益处。2012 年 2 月至 2016 年 12 月，一组 21 名年龄在 22-37 岁（平均 28.0 岁）的患者接受了可生物降解支架的重复阴茎增粗手术。该手术包括插入两个聚乳酸-共-羟基乙酸支架，支架上种植了从阴囊组织标本中制备的实验室成纤维细胞。在此过程中，对第一次手术后形成的组织进行了活检，以进行显微镜分析。平均随访时间为 38 个月。在所有分析的手术切除组织中，均观察到富含纤维组织的纤维结缔组织、小血管和炎症细胞。对这些组织样本的超微结构分析发现，存在大量相互平行排列的胶原纤维，形成束状，并有少数广泛分布的成纤维细胞。第二次手术后，松弛和勃起时的周长平均增加分别为 1.1±0.4cm（范围：0.6-1.7cm）和 1.0±0.3cm（范围：0.6-1.5cm）。使用可生物降解支架进行自体组织工程诱导的新形成组织的显微镜评估显示，存在富含胶原纤维、成纤维细胞和炎症细胞的血管化疏松结缔组织，表明有活跃的新生血管形成和纤维蛋白原形成。重复增强手术的益处具有统计学意义。
