Department of Urology, Tangshan Gongren Hospital, Tangshan, China.
Eur Rev Med Pharmacol Sci. 2018 May;22(10):2985-2990. doi: 10.26355/eurrev_201805_15054.
To investigate the effects of miR-519d on the proliferation, cycle and invasion of human prostate cancer PC3 cells and its possible molecular mechanism.
The proliferation, cycle, and invasion of human prostate cancer PC3 cells were detected via cell counting kit-8 (CCK-8) and transwell assay. The expression of NRBP1 mRNA was detected via reverse transcription-polymerase chain reaction (RT-PCR). Western blotting was used to detect the expression of NRBP1, cyclin D1, and epithelial-mesenchymal transition (EMT) markers.
The expression of miR-519d in prostate cancer was decreased, which was correlated with tumor size, metastasis, and staging. Proliferation, cycle, and invasion of PC3 cells were significantly decreased after overexpression of miR-519d. Bioinformatics analysis and Western blotting showed that there was a potential miR-519d binding site in NRBP1 3'-UTR, and overexpression of miR-519d significantly inhibited the expression of NRBP1. The expression of E-cadherin in PC3 cells overexpressing miR-519d was up-regulated, and the expressions of N-cadherin, cyclin D1, vimentin, fibronectin, and Snail were down-regulated.
MiR-519d can repress the proliferation, cycle, and invasion of prostate cancer PC3 cells by inhibiting NRBP1.
探讨 miR-519d 对人前列腺癌细胞 PC3 增殖、周期和侵袭的影响及其可能的分子机制。
通过细胞计数试剂盒-8（CCK-8）和 Transwell 实验检测人前列腺癌细胞 PC3 增殖、周期和侵袭情况。采用逆转录-聚合酶链反应（RT-PCR）检测 NRBP1mRNA 的表达。Western blot 检测 NRBP1、细胞周期蛋白 D1 和上皮间质转化（EMT）标志物的表达。
miR-519d 在前列腺癌中的表达降低，与肿瘤大小、转移和分期相关。过表达 miR-519d 后 PC3 细胞的增殖、周期和侵袭明显减少。生物信息学分析和 Western blot 表明，NRBP1 3'-UTR 存在潜在的 miR-519d 结合位点，过表达 miR-519d 可显著抑制 NRBP1 的表达。过表达 miR-519d 的 PC3 细胞中 E-钙黏蛋白的表达上调，N-钙黏蛋白、细胞周期蛋白 D1、波形蛋白、纤连蛋白和 Snail 的表达下调。
miR-519d 可通过抑制 NRBP1 抑制前列腺癌细胞 PC3 的增殖、周期和侵袭。
