College of Pharmacy, Graduate School of Pharmaceutical Sciences , Ewha Womans University , Seoul 03760 , Republic of Korea.
Faculté de Pharmacie de Paris , Université Paris Descartes , Paris 75006 , France.
Nano Lett. 2018 Jul 11;18(7):4279-4284. doi: 10.1021/acs.nanolett.8b01267. Epub 2018 Jun 8.
Enzymatic synthesis of RNA nanostructures is achieved by isothermal rolling circle transcription (RCT). Each arm of RNA nanostructures provides a functional role of Dicer substrate RNA inducing sequence specific RNA interference (RNAi). Three different RNAi sequences (GFP, RFP, and BFP) are incorporated within the three-arm junction RNA nanostructures (Y-RNA). The template and helper DNA strands are designed for the large-scale in vitro synthesis of RNA strands to prepare self-assembled Y-RNA. Interestingly, Dicer processing of Y-RNA is highly influenced by its physical structure and different gene silencing activity is achieved depending on its arm length and overhang. In addition, enzymatic synthesis allows the preparation of various Y-RNA structures using a single DNA template offering on demand regulation of multiple target genes.
通过等温滚环转录（RCT）实现 RNA 纳米结构的酶促合成。RNA 纳米结构的每一个臂都提供了 Dicer 底物 RNA 诱导序列特异性 RNA 干扰（RNAi）的功能作用。三个不同的 RNAi 序列（GFP、RFP 和 BFP）被整合到三臂连接 RNA 纳米结构（Y-RNA）中。模板和辅助 DNA 链被设计用于 RNA 链的大规模体外合成，以制备自组装的 Y-RNA。有趣的是，Dicer 对 Y-RNA 的加工受到其物理结构的高度影响，并且根据其臂长和突出端的不同，可以实现不同的基因沉默活性。此外，酶促合成允许使用单个 DNA 模板制备各种 Y-RNA 结构，从而按需调节多个靶基因。
