[2-甲氧基-4-(1-丙烯基)-羟基苯在温血动物机体中的分布特性]

[The peculiar features of the distribution of 2-metoxy-4-(1-propenyl)-hydroxybenzene in the organism of the warm-blooded animals].

作者信息

Shormanov V K, Tsatsua E P, Astashkina A P, Vorob'eva O A, Mastikhina Yu A

机构信息

Department of Pharmaceutical, Toxicological and analytical Chemistry, Kursk State Medical University, Ministry of Health of the Russia, Kursk, Russia, 305041.

Department of Physical and Analytical Chemistry, Institute of Natural Resources, Tomsk National Research Polytechnical University, Tomsk, Russia, 634050.

出版信息

Sud Med Ekspert. 2018;61(3):35-39. doi: 10.17116/sudmed2018613-39.

DOI:10.17116/sudmed2018613-39

PMID:29863718

Abstract

The present study was designed to elucidate the distribution patterns of 2-metoxy-4-(1-propenyl)-hydroxybenzene in the organism of the warm-blooded animals (rats) after its intragastric administration at a dose equivalent to three 50% lethal doses (LD-50) for this compound. 2-metoxy-4-(1-propenyl)-hydroxybenzene was extracted in the unmodified form from the organs and blood of the dead animals after the dualfold infusion of the tissues with ethyl-acetate and purification by chromatography on a macrocolumn with silica gel L 40/100 mcm making use of the hexane-acetone mixture (7:3) mobile phase as the eluent. TLC, HPLC, and UV-spectrometry were employed to identify and quantify the analyte. The study has demonstrated the presence of unmetabolized 2-metoxy-4-(1-propenyl)-hydroxybenzene in the organs and blood of the poisoned animals. The largest amounts of this compound (expressed in milligrams per 100 g of the tissue) were accumulated in the stomach with its contents (236.22±28.21), small intestine with their contents (122.29±15.55), lungs (44.28±2.10), and spleen (44.00±4.70).

摘要

本研究旨在阐明2-甲氧基-4-(1-丙烯基)-羟基苯在温血动物（大鼠）体内的分布模式，该化合物经胃内给药，剂量相当于其三个50%致死剂量（LD-50）。在对组织进行两次乙酸乙酯灌注并使用正己烷-丙酮混合物（7:3）作为流动相在装有硅胶L 40/100 mcm的大柱上进行色谱纯化后，从死亡动物的器官和血液中以未修饰的形式提取2-甲氧基-4-(1-丙烯基)-羟基苯。采用薄层色谱法（TLC）、高效液相色谱法（HPLC）和紫外光谱法对分析物进行鉴定和定量。该研究表明，中毒动物的器官和血液中存在未代谢的2-甲氧基-4-(1-丙烯基)-羟基苯。该化合物的最大含量（以每100克组织中的毫克数表示）积聚在含有内容物的胃（236.22±28.21）、含有内容物的小肠（122.29±15.55）、肺（44.28±2.10）和脾脏（44.00±4.70）中。