Akrigg A, Wilkinson G W, Oram J D
Virus Res. 1985 Mar;2(2):107-21. doi: 10.1016/0168-1702(85)90242-4.
The nucleotide sequence of the major immediate early (IE) gene of human cytomegalovirus strain AD169 was determined. The structure of the gene was examined by nuclease mapping and by sequence analysis of a cDNA clone made from IE mRNA. The gene encodes a spliced molecule of 1736 nucleotides, made up of four exon sequences of 121, 88, 185 and 1342 nucleotides. Three introns (827, 114 and 170n) were located near the 5' end of the gene. A single open reading frame starting in the second exon extends for 491 amino acids, corresponding to a protein of molecular weight 64000. The putative promoter region contains several short direct and inverted repeat sequences of 16, 18, 19 and 21 nucleotides, which extend 509n upstream from the transcription start site. The structure of the major IE gene and its protein product are discussed and compared with the corresponding IE gene from the Towne strain of HCMV.
测定了人巨细胞病毒AD169株主要立即早期（IE）基因的核苷酸序列。通过核酸酶图谱分析以及对由IE mRNA制备的cDNA克隆进行序列分析，对该基因的结构进行了研究。该基因编码一个由1736个核苷酸组成的剪接分子，由四个外显子序列组成，分别为121、88、185和1342个核苷酸。三个内含子（827、114和170个核苷酸）位于基因5'端附近。从第二个外显子开始的一个单一开放阅读框延伸491个氨基酸，对应于一个分子量为64000的蛋白质。推测的启动子区域包含几个16、18、19和21个核苷酸的短直接重复和反向重复序列，它们从转录起始位点向上游延伸509个核苷酸。讨论了主要IE基因的结构及其蛋白质产物，并与HCMV Towne株的相应IE基因进行了比较。
