• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人巨细胞病毒AD169株中由细胞相关序列编码的2.2千碱基早期转录本类别

2.2-kilobase class of early transcripts encoded by cell-related sequences in human cytomegalovirus strain AD169.

作者信息

Staprans S I, Spector D H

出版信息

J Virol. 1986 Feb;57(2):591-602. doi: 10.1128/JVI.57.2.591-602.1986.

DOI:10.1128/JVI.57.2.591-602.1986
PMID:3003391
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC252774/
Abstract

In this report we describe the kinetics of appearance and fine mapping of a 2.2-kilobase (kb) class of transcripts arising from a region of the human cytomegalovirus genome which contains cell-related sequences. These transcripts are encoded by adjacent EcoRI fragments R and d (map units 0.682 to 0.713), located within the long unique segment of the genome. The 2.2-kb RNAs were first detected at 8h postinfection and appeared at comparable or slightly lower levels at 28 and 72 h postinfection. At late times (72 h) additional transcripts were detected with probes from this region. RNase, S1 nuclease, and exonuclease VII protection analyses of 8- and 28-h RNA indicated that the 2.2-kb RNAs had a complex spliced structure consisting of invariable 5' and internal exons and a heterogeneous 3' exon. The position of the 5' end of the RNA was determined with respect to the nucleotide sequence. Analysis of this sequence showed that the cell-related sequences were contained within a long open reading frame in the 5' exon.

摘要

在本报告中，我们描述了源自人巨细胞病毒基因组中一个包含细胞相关序列区域的2.2千碱基（kb）转录本类别的出现动力学及精细定位。这些转录本由位于基因组长独特区段内相邻的EcoRI片段R和d（图谱单位0.682至0.713）编码。2.2 kb的RNA在感染后8小时首次检测到，在感染后28小时和72小时以相当或略低的水平出现。在后期（72小时），用该区域的探针检测到了其他转录本。对8小时和28小时RNA进行的核糖核酸酶、S1核酸酶和核酸外切酶VII保护分析表明，2.2 kb的RNA具有复杂的剪接结构，由不变的5'端外显子和内部外显子以及一个异质的3'端外显子组成。相对于核苷酸序列确定了RNA 5'端的位置。对该序列的分析表明，细胞相关序列包含在5'端外显子的一个长开放阅读框内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/3feb3348174b/jvirol00113-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/9c24177bb0b5/jvirol00113-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/c2ed979d7f58/jvirol00113-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/cbe74fcce502/jvirol00113-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/b3b788e0fe2f/jvirol00113-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/9a5e6cf86b88/jvirol00113-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/3feb3348174b/jvirol00113-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/9c24177bb0b5/jvirol00113-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/c2ed979d7f58/jvirol00113-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/cbe74fcce502/jvirol00113-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/b3b788e0fe2f/jvirol00113-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/9a5e6cf86b88/jvirol00113-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aade/252774/3feb3348174b/jvirol00113-0196-a.jpg

相似文献

1
2.2-kilobase class of early transcripts encoded by cell-related sequences in human cytomegalovirus strain AD169.人巨细胞病毒AD169株中由细胞相关序列编码的2.2千碱基早期转录本类别
J Virol. 1986 Feb;57(2):591-602. doi: 10.1128/JVI.57.2.591-602.1986.
2
Analysis of the major transcripts encoded by the long repeat of human cytomegalovirus strain AD169.人巨细胞病毒AD169株长重复序列编码的主要转录本分析
J Virol. 1985 Mar;53(3):711-8. doi: 10.1128/JVI.53.3.711-718.1985.
3
Posttranscriptional regulation of a class of human cytomegalovirus phosphoproteins encoded by an early transcription unit.由一个早期转录单元编码的一类人巨细胞病毒磷蛋白的转录后调控
J Virol. 1989 Jul;63(7):3117-27. doi: 10.1128/JVI.63.7.3117-3127.1989.
4
Transcription analysis and sequence of the putative murine cytomegalovirus DNA polymerase gene.假定的鼠巨细胞病毒DNA聚合酶基因的转录分析及序列
Virology. 1991 Nov;185(1):169-86. doi: 10.1016/0042-6822(91)90765-4.
5
Structural analysis of the major immediate early gene of human cytomegalovirus.人类巨细胞病毒主要立即早期基因的结构分析
J Virol. 1984 Jan;49(1):190-9. doi: 10.1128/JVI.49.1.190-199.1984.
6
The structure of the major immediate early gene of human cytomegalovirus strain AD169.人巨细胞病毒AD169株主要立即早期基因的结构
Virus Res. 1985 Mar;2(2):107-21. doi: 10.1016/0168-1702(85)90242-4.
7
Identification of sequence requirements and trans-acting functions necessary for regulated expression of a human cytomegalovirus early gene.鉴定人类巨细胞病毒早期基因调控表达所需的序列要求和反式作用功能。
J Virol. 1988 Sep;62(9):3463-73. doi: 10.1128/JVI.62.9.3463-3473.1988.
8
Characterization of human cytomegalovirus UL84 early gene and identification of its putative protein product.人巨细胞病毒UL84早期基因的特征分析及其假定蛋白产物的鉴定。
J Virol. 1992 Feb;66(2):1098-108. doi: 10.1128/JVI.66.2.1098-1108.1992.
9
Four phosphoproteins with common amino termini are encoded by human cytomegalovirus AD169.人类巨细胞病毒AD169编码了四种具有共同氨基末端的磷蛋白。
J Virol. 1988 Jan;62(1):331-40. doi: 10.1128/JVI.62.1.331-340.1988.
10
Analysis of a region of the human cytomegalovirus (AD169) genome coding for a 25-kilodalton virion protein.对人巨细胞病毒（AD169）基因组中编码一种25千道尔顿病毒体蛋白的区域进行分析。
J Virol. 1989 Jan;63(1):233-41. doi: 10.1128/JVI.63.1.233-241.1989.

引用本文的文献

1
Differential Requirement of Human Cytomegalovirus UL112-113 Protein Isoforms for Viral Replication.人巨细胞病毒UL112 - 113蛋白异构体对病毒复制的不同需求
J Virol. 2017 Aug 10;91(17). doi: 10.1128/JVI.00254-17. Print 2017 Sep 1.
2
Mutations in the M112/M113-coding region facilitate murine cytomegalovirus replication in human cells.M112/M113 编码区的突变有助于小鼠巨细胞病毒在人细胞中的复制。
J Virol. 2010 Aug;84(16):7994-8006. doi: 10.1128/JVI.02624-09. Epub 2010 Jun 2.
3
Exon 3 of the human cytomegalovirus major immediate-early region is required for efficient viral gene expression and for cellular cyclin modulation.

本文引用的文献

1
A catalogue of splice junction sequences.剪接连接序列目录。
Nucleic Acids Res. 1982 Jan 22;10(2):459-72. doi: 10.1093/nar/10.2.459.
2
Organization and expression of eucaryotic split genes coding for proteins.编码蛋白质的真核生物断裂基因的组织与表达。
Annu Rev Biochem. 1981;50:349-83. doi: 10.1146/annurev.bi.50.070181.002025.
3
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.真核生物mRNA翻译起始位点上游序列的汇编与分析。
人巨细胞病毒主要立即早期区域的外显子3对于有效的病毒基因表达和细胞周期蛋白调节是必需的。
J Virol. 2005 Jun;79(12):7438-52. doi: 10.1128/JVI.79.12.7438-7452.2005.
4
Absence of IE1 p72 protein function during low-multiplicity infection by human cytomegalovirus results in a broad block to viral delayed-early gene expression.人巨细胞病毒低 multiplicity 感染期间 IE1 p72 蛋白功能缺失导致病毒延迟早期基因表达广泛受阻。
J Virol. 2002 May;76(9):4441-55. doi: 10.1128/jvi.76.9.4441-4455.2002.
5
Separate DNA elements containing ATF/CREB and IE86 binding sites differentially regulate the human cytomegalovirus UL112-113 promoter at early and late times in the infection.含有ATF/CREB和IE86结合位点的独立DNA元件在感染的早期和晚期对人巨细胞病毒UL112 - 113启动子进行差异调控。
J Virol. 1998 Apr;72(4):2697-707. doi: 10.1128/JVI.72.4.2697-2707.1998.
6
The role of ATF in regulating the human cytomegalovirus DNA polymerase (UL54) promoter during viral infection.ATF在病毒感染期间调控人巨细胞病毒DNA聚合酶（UL54）启动子中的作用。
J Virol. 1997 Mar;71(3):2120-6. doi: 10.1128/JVI.71.3.2120-2126.1997.
7
Analysis of the complete DNA sequence of murine cytomegalovirus.小鼠巨细胞病毒全DNA序列分析
J Virol. 1996 Dec;70(12):8833-49. doi: 10.1128/JVI.70.12.8833-8849.1996.
8
CREB and CREB-binding proteins play an important role in the IE2 86-kilodalton protein-mediated transactivation of the human cytomegalovirus 2.2-kilobase RNA promoter.CREB与CREB结合蛋白在人巨细胞病毒2.2千碱基RNA启动子的IE2 86千道尔顿蛋白介导的反式激活中发挥重要作用。
J Virol. 1996 Oct;70(10):6955-66. doi: 10.1128/JVI.70.10.6955-6966.1996.
9
Four of eleven loci required for transient complementation of human cytomegalovirus DNA replication cooperate to activate expression of replication genes.人巨细胞病毒DNA复制瞬时互补所需的11个基因座中的4个协同激活复制基因的表达。
J Virol. 1996 Jan;70(1):383-92. doi: 10.1128/JVI.70.1.383-392.1996.
10
Multiple regulatory events influence human cytomegalovirus DNA polymerase (UL54) expression during viral infection.多种调控事件影响病毒感染期间人巨细胞病毒DNA聚合酶（UL54）的表达。
J Virol. 1996 Jan;70(1):373-82. doi: 10.1128/JVI.70.1.373-382.1996.
Nucleic Acids Res. 1984 Jan 25;12(2):857-72. doi: 10.1093/nar/12.2.857.
4
A detailed analysis of an HSV-1 early promoter: sequences involved in trans-activation by viral immediate-early gene products are not early-gene specific.对单纯疱疹病毒1型早期启动子的详细分析：病毒立即早期基因产物参与反式激活的序列并非早期基因特异性的。
Nucleic Acids Res. 1984 Apr 11;12(7):3037-56. doi: 10.1093/nar/12.7.3037.
5
The oncogenic potential of human cytomegalovirus.
Prog Med Virol. 1984;29:45-89.
6
Predominant immediate-early transcripts of human cytomegalovirus AD 169.人巨细胞病毒AD 169的主要即刻早期转录本
J Virol. 1984 Feb;49(2):363-70. doi: 10.1128/JVI.49.2.363-370.1984.
7
Structural analysis of the major immediate early gene of human cytomegalovirus.人类巨细胞病毒主要立即早期基因的结构分析
J Virol. 1984 Jan;49(1):190-9. doi: 10.1128/JVI.49.1.190-199.1984.
8
Structure of the transforming region of human cytomegalovirus AD169.人巨细胞病毒AD169转化区的结构
J Virol. 1984 Jan;49(1):109-15. doi: 10.1128/JVI.49.1.109-115.1984.
9
Human cytomegalovirus (strain AD169) contains sequences related to the avian retrovirus oncogene v-myc.
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3889-93. doi: 10.1073/pnas.80.13.3889.
10
Neoplastic transformation by a cloned human cytomegalovirus DNA fragment uniquely homologous to one of the transforming regions of herpes simplex virus type 2.由一个克隆的人类巨细胞病毒DNA片段引起的肿瘤转化，该片段与单纯疱疹病毒2型的一个转化区域具有独特的同源性。
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3826-30. doi: 10.1073/pnas.80.12.3826.