Department of Agriculture and Forestry Sciences (DAFNE), University of Tuscia , Viterbo , Italy.
Department of Serology, Zooprophylaxis and Research Institute of Latium and Tuscany M. Aleandri , Rome , Italy.
Nat Prod Res. 2019 Dec;33(24):3465-3471. doi: 10.1080/14786419.2018.1481841. Epub 2018 Jun 4.
is responsible for the cutaneous and visceral form of this zoonotic disease, which is potentially lethal for humans and has dogs as natural reservoir. In the light of the antiparasitic properties displayed by several natural products, promastigotes were exposed to green tea () leaves extract (GTE) and pomegranate () peel extract (PPE). Both extracts, characterized by NMR and HPLC analysis, inhibited parasite proliferation in a dose-dependent manner, as proved by IC evaluation determined by MTT assay.Moreover, the reversibility assay showed that GTE and PPE have an aptotosis-mediated leishmanicidal effect, as evidenced by DNA degradation and confirmed by DNA fragmentation and real-time PCR analyses. Finally, for the first time morphological and ultrastructural alterations induced by a extract on were shown by the use of light, transmission and scanning electron microscopy.
负责这种人畜共患疾病的皮肤和内脏形式，对人类具有潜在致命性，狗是其自然宿主。鉴于几种天然产物表现出的抗寄生虫特性，用绿茶（）叶提取物（GTE）和石榴（）皮提取物（PPE）处理前鞭毛体。这两种提取物均通过 NMR 和 HPLC 分析进行了表征，正如通过 MTT 测定法确定的 IC 评价所证明的那样，它们以剂量依赖的方式抑制寄生虫的增殖。此外，逆转试验表明，GTE 和 PPE 具有诱导细胞凋亡的杀利什曼原虫作用，这一点可通过 DNA 降解得到证明，并通过 DNA 片段化和实时 PCR 分析得到证实。最后，首次通过使用光、透射和扫描电子显微镜观察到提取物对的形态和超微结构的改变。
