Department of Molecular Biology, National AIDS Research Institute, Pune, 411026, India.
Department of Clinical Sciences, National AIDS Research Institute, Pune, 411026, India.
Microb Pathog. 2018 Aug;121:325-330. doi: 10.1016/j.micpath.2018.06.001. Epub 2018 Jun 2.
The genetic variations in APOBEC3G gene are correlated with HIV disease progression. These variations differ in different ethnic groups. The prevalence of APOBEC3G (-90C/G, -571G/C) variations have not been studied in Indian population. Hence, we assessed the occurrence of APOBEC3G polymorphisms in HIV patients and its association with acquisition of HIV and disease progression. Polymorphisms in APOBEC3G were genotyped in a total of 153 HIV patients, naïve to ARV and 156 healthy controls by PCR-RFLP method. In single locus model, the frequency of distribution of APOBEC3G -90CG, -571 GC genotypes were higher in HIV patients as compared to healthy controls (57.5% vs. 50.0%, OR = 1.22; 17.0% vs. 12.8%, OR = 1.39). In double locus model, the dominant -571 GC + CC genotype was distributed at a much higher frequency in HIV patients as compared to healthy controls (18.3% vs. 14.1%, OR = 1.50). The frequency of APOBEC3G -571CC and CC + GC genotypes were higher in early HIV disease stage as compared to healthy controls (23.9% vs. 12.8%, OR = 2.23, P = 0.08; 28.3% vs. 14.1%, OR = 2.40, P = 0.04). APOBEC3G-571 GC and GC + CC genotypes were more prevalent in HIV patients consuming tobacco and alcohol as compared to non-users (22.7% vs. 15.3%, OR = 1.71, P = 0.56; 27.3% vs. 16.5%, OR = 1.90, P = 0.39 and 31.6% vs. 13.6%, OR = 2.31, P = 0.08; 36.8% vs14.8%, OR = 2.49, P = 0.04, respectively). In conclusion, APOBEC3G-571G/C polymorphism was associated with the early stage of HIV infection and could potentially influence HIV disease progression in alcohol users. The distribution of APOBEC3G polymorphisms and its haplotypes were not significantly different between HIV patients and healthy controls.
APOBEC3G 基因的遗传变异与 HIV 疾病进展相关。这些变异在不同的种族群体中存在差异。APOBEC3G（-90C/G、-571G/C）变异在印度人群中的流行情况尚未得到研究。因此，我们评估了 HIV 患者中 APOBEC3G 多态性的发生情况及其与 HIV 获得和疾病进展的关系。通过 PCR-RFLP 方法，对 153 名未经 ARV 治疗的 HIV 患者和 156 名健康对照者的 APOBEC3G 多态性进行了基因分型。在单基因座模型中，与健康对照组相比，HIV 患者 APOBEC3G-90CG、-571GC 基因型的分布频率更高（57.5%比 50.0%，OR=1.22；17.0%比 12.8%，OR=1.39）。在双基因座模型中，与健康对照组相比，HIV 患者中显性-571GC+CC 基因型的分布频率更高（18.3%比 14.1%，OR=1.50）。与健康对照组相比，早期 HIV 疾病阶段 APOBEC3G-571CC 和 CC+GC 基因型的频率更高（23.9%比 12.8%，OR=2.23，P=0.08；28.3%比 14.1%，OR=2.40，P=0.04）。与非使用者相比，吸烟和饮酒的 HIV 患者中 APOBEC3G-571GC 和 GC+CC 基因型更为常见（22.7%比 15.3%，OR=1.71，P=0.56；27.3%比 16.5%，OR=1.90，P=0.39；31.6%比 13.6%，OR=2.31，P=0.08；36.8%比 14.8%，OR=2.49，P=0.04）。总之，APOBEC3G-571G/C 多态性与 HIV 感染的早期阶段相关，可能影响酒精使用者的 HIV 疾病进展。HIV 患者与健康对照组之间 APOBEC3G 多态性及其单倍型的分布无显著差异。
