Iqbal Khurshid, Imran Muhammad, Ullah Shafi, Jamal Muhsin, Waheed Yasir
Department of Medical Laboratory Sciences, Imperial College of Business Studies, Lahore, Pakistan.
Department of Microbiology, University of Health Sciences Lahore, Pakistan.
Curr HIV Res. 2018;16(4):297-301. doi: 10.2174/1570162X16666181018155827.
Human immunodeficiency virus (HIV) infection is a global health burden which ultimately results in acquired immune deficiency syndrome (AIDS). There are multiple host factors which are capable of limiting HIV-1 replication. One of the most important host factors which inhibit HIV-1 DNA synthesis is the apolipoprotein B mRNA-editing enzyme, catalytic polypeptide- like 3G (APOBEC3G). Any genetic variation of this important host factor may influence the host susceptibility to viral infection.
The aim of the current study was to evaluate any correlation of APOBEC3G genetic variation rs8177832 with HIV-1 infection.
The study involved 142 healthy control and 100 HIV-1 infected subjects. The genetic variation rs8177832 of all studied subjects was determined by allele-specific polymerase chain reaction (AS-PCR).
The results showed that the distribution of rs8177832 genotypes AA, AG and GG in healthy subjects and HIV-1 subjects was; 42.253%, 42.957%, 14.788% and 66%, 27%, 7% respectively. Statistical analyses of data showed that there was a significant variation in rs8177832 genotype AA in healthy control and HIV-1 infected subjects (42.257% vs 66%; p-value<0.001).
Thus it was concluded that APOBEC3G rs8177832 AA genotype contributes in genetic predisposition to HIV-1 infection in Pakistani population.
人类免疫缺陷病毒（HIV）感染是一项全球性的健康负担，最终会导致获得性免疫缺陷综合征（AIDS）。有多种宿主因素能够限制HIV-1复制。抑制HIV-1 DNA合成的最重要宿主因素之一是载脂蛋白B mRNA编辑酶催化多肽样3G（APOBEC3G）。这一重要宿主因素的任何基因变异都可能影响宿主对病毒感染的易感性。
本研究旨在评估APOBEC3G基因变异rs8177832与HIV-1感染之间的相关性。
该研究纳入了142名健康对照者和100名HIV-1感染者。通过等位基因特异性聚合酶链反应（AS-PCR）确定所有研究对象的基因变异rs8177832。
结果显示，健康受试者和HIV-1感染者中rs8177832基因型AA、AG和GG的分布分别为42.253%、42.957%、14.788%和66%、27%、7%。数据的统计分析表明，健康对照者和HIV-1感染者中rs8177832基因型AA存在显著差异（42.257%对66%；p值<0.001）。
因此得出结论，在巴基斯坦人群中，APOBEC3G rs8177832 AA基因型在HIV-1感染的遗传易感性中起作用。
