Biotec BetaGlucans AS, 9019 Tromsø, Norway; Drug Transport and Delivery Research Group, Department of Pharmacy, Faculty of Health Sciences, University of Tromsø The Arctic University of Norway, 9037 Tromsø, Norway.
Biotec BetaGlucans AS, 9019 Tromsø, Norway.
Eur J Pharm Sci. 2018 Aug 30;121:269-280. doi: 10.1016/j.ejps.2018.05.031. Epub 2018 Jun 1.
The increased prevalence of chronic wounds requires novel treatment options. The aim of this study was to develop a beta-glucan (βG)-loaded nanofiber wound dressing. Nanofibers were prepared using the needle-free Nanospider™ technology, an electrospinning method which enables the production of nanofibers at an industrial scale. The βG was selected as active ingredient based on its confirmed wound healing potential in both animals and humans. Hydroxypropyl methylcellulose (HPMC) and polyethylene oxide (PEO) were included as copolymers. Rheological profiles of spinning solutions containing HPMC, PEO, βG, ethanol and water, were optimized. The nanofiber formation was confirmed by Field Emission Scanning Electron Microscopy (FE-SEM), and both nanofibers with (βG-nanofibers) or without βG (NoβG-nanofibers) were evaluated by their swelling index and FT-IR spectroscopy. The formulations, active ingredient and excipients were tested for their possible in vitro toxicity in keratinocytes. Finally, the wound healing potential of the nanofibers was tested in externally induced excisional wounds in male diabetic db/db mice. Three different doses of βG-nanofibers and the βG-free, NoβG-nanofibers, were evaluated for their in vivo wound healing efficacy. All nanofiber-treatments provided improved wound healing as compared to the negative control (water). All βG-nanofiber treated groups exhibited significantly improved wound healing as compared to the NoβG-nanofiber treated group, indicating the potential of βG-nanofibers as wound dressing.
慢性伤口的患病率不断上升，这就需要新的治疗方法。本研究旨在开发一种负载β-葡聚糖（βG）的纳米纤维创伤敷料。纳米纤维是使用无针 Nanospider™技术制备的，这是一种静电纺丝方法，可以在工业规模上生产纳米纤维。选择βG作为活性成分是基于其在动物和人类中已被证实的伤口愈合潜力。羟丙基甲基纤维素（HPMC）和聚氧化乙烯（PEO）被包含为共聚物。优化了含有 HPMC、PEO、βG、乙醇和水的纺丝溶液的流变特性。通过场发射扫描电子显微镜（FE-SEM）确认了纳米纤维的形成，并且对含有βG 的纳米纤维（βG-纳米纤维）和不含βG 的纳米纤维（NoβG-纳米纤维）进行了评价，评价方法为溶胀指数和傅里叶变换红外光谱（FT-IR）分析。对制剂、活性成分和赋形剂进行了角质形成细胞体外毒性测试。最后，在雄性糖尿病 db/db 小鼠的外部诱导性切除伤口中测试了纳米纤维的伤口愈合潜力。评估了三种不同剂量的βG-纳米纤维和无βG 的纳米纤维（NoβG-纳米纤维）的体内伤口愈合效果。与阴性对照（水）相比，所有纳米纤维处理均能改善伤口愈合。与 NoβG-纳米纤维处理组相比，所有βG-纳米纤维处理组的伤口愈合效果均有显著改善，表明βG-纳米纤维作为伤口敷料具有潜力。
