Manakhov Anton M, Permyakova Elizaveta S, Solovieva Anastasiya O, Sitnikova Natalya A, Kiryukhantsev-Korneev Philipp V, Konopatsky Anton S, Shtansky Dmitry V
Research Institute of Clinical and Experimental Lymphology-Branch of the ICG SB RAS, 2 Timakova St., 630060 Novosibirsk, Russia.
National University of Science and Technology "MISiS", Leninsky Prospekt 4, 119049 Moscow, Russia.
Polymers (Basel). 2023 Mar 14;15(6):1440. doi: 10.3390/polym15061440.
Platelet-rich Plasma (PRP) is an ensemble of growth factors, extracellular matrix components, and proteoglycans that are naturally balanced in the human body. In this study, the immobilization and release of PRP component nanofiber surfaces modified by plasma treatment in a gas discharge have been investigated for the first time. The plasma-treated polycaprolactone (PCL) nanofibers were utilized as substrates for the immobilization of PRP, and the amount of PRP immobilized was assessed by fitting a specific X-ray Photoelectron Spectroscopy (XPS) curve to the elemental composition changes. The release of PRP was then revealed by measuring the XPS after soaking nanofibers containing immobilized PRP in buffers of varying pHs (4.8; 7.4; 8.1). Our investigations have proven that the immobilized PRP would continue to cover approximately fifty percent of the surface after eight days.
富含血小板血浆（PRP）是一组生长因子、细胞外基质成分和蛋白聚糖，它们在人体中自然保持平衡。在本研究中，首次研究了通过气体放电等离子体处理改性的PRP成分纳米纤维表面的固定化和释放情况。经等离子体处理的聚己内酯（PCL）纳米纤维被用作固定PRP的底物，通过将特定的X射线光电子能谱（XPS）曲线拟合到元素组成变化来评估固定的PRP量。然后，通过测量将含有固定PRP的纳米纤维浸泡在不同pH值（4.8；7.4；8.1）的缓冲液中后的XPS来揭示PRP的释放情况。我们的研究证明，固定的PRP在八天后仍将继续覆盖约50%的表面。
