Curtis Sherill K, Benner David B, Musil George
Departments of Biophysics, Johnson City, Tennessee 37614.
Quillen-Dishner College of Medicine and Department of Biological Sciences, East Tennessee State University Johnson City, Tennessee 37614.
J Morphol. 1989 Apr;200(1):47-61. doi: 10.1002/jmor.1052000107.
When viewed by scanning electron microscopy (SEM), the spermatozoon of the phorid dipteran Megaselia scalaris appears threadlike, lacking distinct head and tail areas. These areas can be observed, however, in appropriately stained material. Measurements of Feulgen-stained material reveal average lengths of the head, tail, and total cell of 18.7, 128.7, and 147.4 μm, respectively. When tested for sulfhydryl and disulfide groups, the head displays only disulfide groups. Transmission electron microscopy (TEM) reveals 12 different regions: three (1-3) in the head, four (9-12) in the tail, and five (4-8) in a short zone of overlap between the head and tail. Most of the cell lies in regions 9 and 11 of the tail and 3 of the head, accounting for, respectively, 37.3%, 45.7%, and 11.2% of the total length. A tubelike acrosome indents the anterior end of the nucleus. The tail originates asymmetrically in relation to the long axis of the cell as a peglike structure associated with the dorsolateral region of the nucleus. No centriole is visible, and the nucleus has a notched appearance in longitudinal sections. Two mitochondrial derivatives and an axoneme displaying a 9+9+2 microtubule configuration and ATPase activity extend throughout most of the tail. In regions 9 and 10, an asymmetrically arranged accessory body is also present. Features having possible taxonomic utility include the asymmetrically arranged accessory body, the size and shape of the acrosome, and the notched appearance of the nucleus. The present report is apparently the first to describe the spermatozoon of a cyclorrhaphous dipteran which is not a member of the Schizophora.
通过扫描电子显微镜（SEM）观察，蚤蝇科双翅目昆虫扁足蝇的精子呈丝状，没有明显的头部和尾部区域。然而，在经过适当染色的材料中可以观察到这些区域。对福尔根染色材料的测量显示，头部、尾部和整个细胞的平均长度分别为18.7μm、128.7μm和147.4μm。对巯基和二硫键进行检测时，头部仅显示二硫键。透射电子显微镜（TEM）揭示了12个不同的区域：头部有3个（1 - 3），尾部有4个（9 - 12），头部和尾部之间的短重叠区域有5个（4 - 8）。细胞的大部分位于尾部的9区和11区以及头部的3区，分别占总长度的37.3%、45.7%和11.2%。一个管状顶体嵌入细胞核的前端。尾部相对于细胞的长轴不对称地起源，是与细胞核背外侧区域相关的钉状结构。看不到中心粒，细胞核在纵切面上有缺口外观。两个线粒体衍生物和一个显示9 + 9 + 2微管结构和ATP酶活性的轴丝贯穿尾部的大部分区域。在9区和10区，还存在一个不对称排列的附属体。具有可能分类学用途的特征包括不对称排列的附属体、顶体的大小和形状以及细胞核的缺口外观。本报告显然是首次描述非裂翅目的环裂亚目双翅目昆虫的精子。
