Department of Medical Bioscience, University of the Western Cape, Private Bag X17, Bellville, 7535, South Africa.
National Health Laboratory Service, Anatomical Pathology, Tygerberg Hospital, Parow, 7505, South Africa.
Tissue Cell. 2019 Aug;59:44-50. doi: 10.1016/j.tice.2019.06.002. Epub 2019 Jun 22.
Sperm structure and ultrastructure of Hermetia illucens was determined by light microscopy and transmission electron microscopy. The main sperm components were similar as for other Dipteran subspecies, while the ultrastructure revealed distinguishing features in the zone of overlap and anterior flagellar region. Sperm varied in size indicating sperm polymorphism. The head region is lacking an acrosome. The zone of overlap consisted of uniquely organized centriolar adjunct material, partly forming electron dense areas to finally form an outer ring separating the mitochondrial derivatives from the 9 + 9 + 2 axoneme. Accessory bodies arising from the zone of overlap are flanked by smaller to large mitochondrial derivatives into the anterior flagellum. This study confirms sperm structure diversity between brachyceran subspecies and support its relationship with nematoceran subspecies.
通过光学显微镜和透射电子显微镜观察，确定了 Hermetia illucens 的精子结构和超微结构。主要精子成分与其他双翅目亚种相似，而超微结构在前鞭毛区和重叠区揭示了区别特征。精子大小不同，表明存在精子多态性。头部区域缺乏顶体。重叠区由独特组织的中心粒附属物组成，部分形成电子致密区，最终形成一个外环，将线粒体衍生物与 9+9+2 轴丝分开。从中部重叠区产生的附属体被较小到较大的线粒体衍生物包围到前鞭毛。本研究证实了短角亚目亚种之间的精子结构多样性，并支持其与刺尾亚目亚种的关系。
