Lloyd R V, Wilson B S, Kovacs K, Ryan N
Arch Pathol Lab Med. 1985 Jun;109(6):515-7.
Chromogranin was demonstrated by immunohistochemistry in the cytoplasm of human beta-thyrotropin, human beta-follicle-stimulating hormone-, human beta-luteinizing hormone-, and human alpha-subunit-containing cells of the non tumorous human adenohypophyses. Some surgically removed human beta-thyrotropin-, human beta-follicle-stimulating hormone-, human beta-luteinzing hormone-, and human alpha-subunit-producing pituitary adenomas, as well as some null cell-adenomas, exhibited chromogranin immunoreactivity, whereas adenomas storing human growth hormone, human prolactin, or corticotropin were negative. Chromogranin immunopositivity was variable in extent and intensity; not every glycoprotein-producing cell could be immunostained in the nontumorous adenohypophysis and the majority of chromogranin-containing adenomas showed only focal positivity. No explanation can be offered for this variability. The demonstration of chromogranin by the avidin-biotin-peroxidase technique may be helpful in the immunohistochemical characterization of some glycoprotein hormone-producing pituitary adenomas, as well as null-cell adenomas of the human pituitary.
通过免疫组织化学法在人非肿瘤性腺垂体中含人促甲状腺激素β亚基、人促卵泡激素β亚基、人促黄体生成素β亚基及人α亚基的细胞胞质中证实了嗜铬粒蛋白的存在。一些手术切除的产生人促甲状腺激素β亚基、人促卵泡激素β亚基、人促黄体生成素β亚基及人α亚基的垂体腺瘤，以及一些无功能性细胞腺瘤，呈现嗜铬粒蛋白免疫反应性，而储存人生长激素、人催乳素或促肾上腺皮质激素的腺瘤则呈阴性。嗜铬粒蛋白免疫阳性在范围和强度上存在差异；在非肿瘤性腺垂体中并非每个产生糖蛋白的细胞都能被免疫染色，且大多数含嗜铬粒蛋白的腺瘤仅表现为局灶性阳性。无法对这种变异性作出解释。抗生物素蛋白-生物素-过氧化物酶技术对嗜铬粒蛋白的证实可能有助于某些产生糖蛋白激素的垂体腺瘤以及人垂体无功能性细胞腺瘤的免疫组织化学特征鉴定。
