Watanabe T, Hayashi Y, Ogasawara N, Tomoita T
Biochem Genet. 1985 Feb;23(1-2):105-13. doi: 10.1007/BF00499116.
Restriction endonuclease cleavage patterns of mitochondrial DNA (mtDNA) of pigs and Japanese wild boars were analyzed using 17 enzymes which recognize six nucleotides. The map of cleavage sites was made by double-digestion methods. Polymophism of mtDNA was detected in the digestion by BglII, EcoRV, ScaI, and StuI. The restriction cleavage patterns were identical among the breeds of Landrace, Hampshire, Duroc I, and Large White I (A type). The patterns of Large White II were the same as those of Japanese wild boars (B type). A difference between the A type and the B type of mtDNA was found in the case of three restriction enzymes, BglII, ScaI, and StuI, and the nucleotide alterations between them were estimated as more than six. On the other hand, a difference between mtDNA from almost all pigs and mtDNA from Duroc II was detected using EcoRV. We suggest that the difference of mtDNA between the A type and the B type of mtDNA could result from the different origin of boars, that is, whether they were of European or Asian origin.
使用17种识别六核苷酸的酶分析了猪和日本野猪线粒体DNA（mtDNA）的限制性内切酶切割模式。通过双酶切方法绘制了切割位点图谱。在BglII、EcoRV、ScaI和StuI酶切中检测到mtDNA的多态性。长白猪、汉普夏猪、杜洛克I和大白猪I品种（A型）的限制性切割模式相同。大白猪II的模式与日本野猪的模式相同（B型）。在BglII、ScaI和StuI这三种限制性酶的情况下，发现了A型和B型mtDNA之间的差异，估计它们之间的核苷酸改变超过六个。另一方面，使用EcoRV检测到几乎所有猪的mtDNA与杜洛克II的mtDNA之间存在差异。我们认为，A型和B型mtDNA之间的差异可能源于野猪的不同起源，即它们是欧洲起源还是亚洲起源。
