Umenishi F, Han B K, Ikemoto S
Department of Legal Medicine and Human Genetics, Jichi Medical School, Tochigi-ken, Japan.
J Vet Med Sci. 1993 Apr;55(2):313-7. doi: 10.1292/jvms.55.313.
Mitochondrial DNA (mtDNA) polymorphism was studied in 21 Jindo dogs inhabiting Jin Island off the Korean peninsula. The polymorphism was analyzed with 10 restriction endonucleases that recognize six base pairs. The sizes of the mtDNA fragments produced by digestion using each endonucleases were separated by agarose gel electrophoresis, and the polymorphisms were detected with Japanese mongrel dog mtDNA as a probe. The mtDNA polymorphism in Jindo dogs was observed with four restriction endonucleases, Apa I, EcoR V, Hinc II, and Sty I. However, no polymorphism was detected with BamH I, Bgl II, EcoR I, Hind III, Pst I, or Xba I. The observed restriction endonuclease morphs were classified into 4 types of distinct cleavage patterns. The average number of nucleotide substitutions per nucleotide site in Jindodogs was estimated to be 0.0086. By UPG phylogenetic analysis, the 4 mtDNA types showed only one cluster. This suggests that Jindo dogs have not diverged from the other cluster up to the present and the species is considerably pure.
对居住在朝鲜半岛附近珍岛的21只珍岛犬的线粒体DNA（mtDNA）多态性进行了研究。使用识别六个碱基对的10种限制性内切酶分析了多态性。用每种内切酶消化产生的mtDNA片段大小通过琼脂糖凝胶电泳分离，并用日本杂种犬mtDNA作为探针检测多态性。在珍岛犬中观察到用四种限制性内切酶Apa I、EcoR V、Hinc II和Sty I产生的mtDNA多态性。然而，用BamH I、Bgl II、EcoR I、Hind III、Pst I或Xba I未检测到多态性。观察到的限制性内切酶形态被分为4种不同的切割模式。珍岛犬每个核苷酸位点的核苷酸替代平均数量估计为0.0086。通过UPG系统发育分析，4种mtDNA类型仅显示出一个聚类。这表明珍岛犬至今尚未从其他聚类中分化出来，该物种相当纯正。
